ArticleTransgenic research1997
Promoter/leader deletion analysis and plant expression vectors with the figwort mosaic virus (FMV) full length transcript (FLt) promoter containing single or double enhancer domains.
Article in Transgenic research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 94 citations in OpenAlex.
- A study on the influence of different promoter and 5'UTR (URM) cassettes from Arabidopsis thaliana on the expression level of the reporter gene β glucuronidase in tobacco and cotton.Transgenic research · 2014Pooled it
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- Further Characterization of MUAS35SCP and FUAS35SCP Recombinant Promoters and Their Implication in Translational Research.Molecular biotechnology · 2022Article
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- Insights Into Genetic and Molecular Elements for Transgenic Crop Development.Frontiers in plant science · 2020Article
- A study on optimization of pat gene expression cassette for maize transformation.Molecular biology reports · 2019Article
- Whitefly and aphid inducible promoters of Arabidopsis thaliana L.Journal of genetics · 2018Article
- A Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants.The Plant cell · 2017Article
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- Synthetic promoters in planta.Planta · 2015Review
- Characterization and comparative analysis of promoters from three plant pararetroviruses associated with Dahlia (Dahlia variabilis).Virus genes · 2015Article
- Identification and use of the sugarcane bacilliform virus enhancer in transgenic maize.BMC plant biology · 2014Article
- Comparative analysis of synthetic DNA promoters for high-level gene expression in plants.Planta · 2014Article
- Design and construction of an in-plant activation cassette for transgene expression and recombinant protein production in plants.Nature protocols · 2014Article
- Efficient chimeric plant promoters derived from plant infecting viral promoter sequences.Planta · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The boundaries required for maximal expression from the promoter/leader region of the full length transcript of figwort mosaic virus (FLt promoter) coupled to reporter genes were defined by 5' and 3' deletion analyses. In transient expression assays using protoplasts of Nicotiana edwardsonii, a 314 bp FLt promoter fragment sequence (-249 to +65 from the transcription start site) was sufficient for strong expression activity. Plant expression vectors developed with modified FLt promoters were tested with GUS or CAT as reporter genes in transgenic plants. The FLt promoter is a strong constitutive promoter, with strength comparable to or greater than that of the CaMV 35S promoter. The FLt promoter with its double enhancer domain linked to GUS or CAT reporter genes provides an average 4-fold greater activity than the FLt promoter with a single enhancer domain (-55 to -249 bp upstream fragment) in tests with transgenic plants and in protoplast transient expression assays.
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