ArticleThe Journal of cell biology1997
Sequential actions of Rab5 and Rab7 regulate endocytosis in the Xenopus oocyte.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 67 citations in OpenAlex.
- Modulation of host Rab GTPases by Salmonella: mechanisms of immune evasion and intracellular replication.Molecular biology reports · 2025Review
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- Leishmania highjack host lipid body for its proliferation in macrophages by overexpressing host Rab18 and TRAPPC9 by downregulating miR-1914-3p expression.PLoS pathogens · 2024Article
- Hemoglobin Endocytosis and Intracellular Trafficking: A Novel Way of Heme Acquisition byPathogens (Basel, Switzerland) · 2022Review
- RAB7 activity is required for the regulation of mitophagy in oocyte meiosis and oocyte quality control during ovarian aging.Autophagy · 2022Article
- Lysosomal Functions in Glia Associated with Neurodegeneration.Biomolecules · 2021Review
- SKIP-HOPS recruits TBC1D15 for a Rab7-to-Arl8b identity switch to control late endosome transport.The EMBO journal · 2020Article
- Dysregulation of neurotrophin signaling in the pathogenesis of Alzheimer disease and of Alzheimer disease in Down syndrome.Free radical biology & medicine · 2018Review
- Leishmania donovani resides in modified early endosomes by upregulating Rab5a expression via the downregulation of miR-494.PLoS pathogens · 2017Article
- Rab5 Isoforms Specifically Regulate Different Modes of Endocytosis in Leishmania.The Journal of biological chemistry · 2016Article
- Identification and Characterization of a Novel Broad-Spectrum Virus Entry Inhibitor.Journal of virology · 2016Article
- Functional Characterization of Monomeric GTPase Rab1 in the Secretory Pathway of Leishmania.The Journal of biological chemistry · 2015Article
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- Quantitative proteomics analysis of inborn errors of cholesterol synthesis: identification of altered metabolic pathways in DHCR7 and SC5D deficiency.Molecular & cellular proteomics : MCP · 2010Article
- Rab7 regulates late endocytic trafficking downstream of multivesicular body biogenesis and cargo sequestration.The Journal of biological chemistry · 2009Article
- Leishmania requires Rab7-mediated degradation of endocytosed hemoglobin for their growth.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Late endosomal traffic of the epidermal growth factor receptor ensures spatial and temporal fidelity of mitogen-activated protein kinase signaling.Molecular biology of the cell · 2007Article
- IL-6 and IL-12 specifically regulate the expression of Rab5 and Rab7 via distinct signaling pathways.The EMBO journal · 2006Article
- The small GTPase Rab7 controls the endosomal trafficking and neuritogenic signaling of the nerve growth factor receptor TrkA.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To explore the role of GTPases in endocytosis, we developed an assay using Xenopus oocytes injected with recombinant proteins to follow the uptake of the fluid phase marker HRP. HRP uptake was inhibited in cells injected with GTPgammaS or incubated with aluminum fluoride, suggesting a general role for GTPases in endocytosis. Injection of Rab5 into oocytes, as well as Rab5:Q79L, a mutant with decreased GTPase activity, increased HRP uptake. Injection of Rab5:S34N, the dominant-negative mutant, inhibited HRP uptake. Injection of N-ethylmaleimide-sensitive factor (NSF) stimulated HRP uptake, and ATPase-defective NSF mutants inhibited HRP uptake when coinjected with Rab5:Q79L, confirming a requirement for NSF in endocytosis. Surprisingly, injection of Rab7:WT stimulated both uptake and degradation/activation of HRP. The latter appears to be due to enhanced transport to a late endosomal/prelysosomal degradative compartment that is monensin sensitive. Enhancement of uptake by Rab7 appears to function via an Rab5-sensitive pathway in oocytes since the stimulatory effect of Rab7 was blocked by coinjection of Rab5:S34N. Stimulation of uptake by Rab5 was blocked by Rab5:S34N but not by Rab7:T22N. Our results suggest that Rab7, while functioning downstream of Rab5, may be rate limiting for endocytosis in oocytes.
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