ArticleBiophysical journal1997
Molecular distances from dipolar coupled spin-labels: the global analysis of multifrequency continuous wave electron paramagnetic resonance data.
Article in Biophysical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 142 citations in OpenAlex.
- Advances in EPR Approaches for Studying Structural Properties of Membrane Proteins.Magnetic resonance in chemistry : MRC · 2026Review
- Protein functional dynamics from the rigorous global analysis of DEER data: Conditions, components, and conformations.The Journal of general physiology · 2021Article
- The distance between g-tensors of nitroxide biradicals governs MAS-DNP performance: The case of the bTurea family.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2021Article
- Site-selective labeling and electron paramagnetic resonance studies of human cannabinoid receptor CBBiochimica et biophysica acta. Biomembranes · 2021Article
- Electron Paramagnetic Resonance as a Tool for Studying Membrane Proteins.Biomolecules · 2020Review
- De novo prediction of cross-effect efficiency for magic angle spinning dynamic nuclear polarization.Physical chemistry chemical physics : PCCP · 2019Article
- Conformation of bis-nitroxide polarizing agents by multi-frequency EPR spectroscopy.Physical chemistry chemical physics : PCCP · 2018Article
- Site-Directed Spin Labeling EPR for Studying Membrane Proteins.BioMed research international · 2018Review
- Efficient cross-effect dynamic nuclear polarization without depolarization in high-resolution MAS NMR.Chemical science · 2017Article
- A Straightforward Approach to the Analysis of Double Electron-Electron Resonance Data.Methods in enzymology · 2015Article
- CW dipolar broadening EPR spectroscopy and mechanically aligned bilayers used to measure distance and relative orientation between two TOAC spin labels on an antimicrobial peptide.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2014Article
- Automated structure refinement for a protein heterodimer complex using limited EPR spectroscopic data and a rigid-body docking algorithm: a three-dimensional model for an ankyrin-CDB3 complex.The journal of physical chemistry. B · 2014Article
- Organization of the mitochondrial apoptotic BAK pore: oligomerization of the BAK homodimers.The Journal of biological chemistry · 2014Article
- Use of electron paramagnetic resonance to solve biochemical problems.Biochemistry · 2013Review
- Structural arrangement of the intracellular Ca2+ binding domains of the cardiac Na+/Ca2+ exchanger (NCX1.1): effects of Ca2+ binding.The Journal of biological chemistry · 2013Article
- Inter-spin distance determination using L-band (1-2 GHz) non-adiabatic rapid sweep electron paramagnetic resonance (NARS EPR).Journal of magnetic resonance (San Diego, Calif. : 1997) · 2012Article
- The global analysis of DEER data.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2012Article
- Distance measurements on a dual-labeled TOAC AChR M2δ peptide in mechanically aligned DMPC bilayers via dipolar broadening CW-EPR spectroscopy.The journal of physical chemistry. B · 2012Article
- What site-directed labeling studies tell us about the mechanism of rhodopsin activation and G-protein binding.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2010Review
- Protein structural dynamics revealed by site-directed spin labeling and multifrequency EPR.Biophysical reviews · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
For immobilized nitroxide spin-labels with a well-defined interprobe geometry, resolved dipolar splittings can be observed in continuous wave electron paramagnetic resonance (CW-EPR) spectra for interelectron distances as large as 30 A using perdeuterated probes. In this work, algorithms are developed for calculating CW-EPR spectra of immobilized, dipolar coupled nitroxides, and then used to define the limits of sensitivity to the interelectron distance as a function of geometry and microwave frequency. Secondly, the CW-EPR spectra of N epsilon-spin-labeled coenzyme NAD+ bound to microcrystalline, tetrameric glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been collected at 9.8, 34, and 94 GHz. These data have been analyzed, using a combination of simulated annealing and global analysis, to obtain a unique fit to the data. The values of the intermitroxide distance and the five angles defining the relative orientation of the two nitroxides are in reasonable agreement with a molecular model built from the known crystal structure. Finally, the effect of rigid body isotropic rotational diffusion on the CW-EPR spectra of dipolar coupled nitroxides has been investigated using an algorithm based on Brownian dynamics trajectories. These calculations demonstrate the sensitivity of CW-EPR spectra to dipolar coupling in the presence of rigid body rotational diffusion.
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