ArticleBiophysical journal1997
An optimized approach to membrane capacitance estimation using dual-frequency excitation.
Article in Biophysical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Transient capacitance changes recorded from vestibular type I hair cells are produced by GThe Journal of physiology · 2025Article
- Scientist and data architect collaborate to curate and archive an inner ear electrophysiology data collection.PloS one · 2019Article
- High resolution electrophysiological techniques for the study of calcium-activated exocytosis.Biochimica et biophysica acta · 2012Review
- Power efficiency of outer hair cell somatic electromotility.PLoS computational biology · 2009Article
- Membrane composition modulates prestin-associated charge movement.The Journal of biological chemistry · 2008Article
- Functional expression and microdomain localization of prestin in cultured cells.Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2007Article
- Voltage-dependent capacitance of human embryonic kidney cells.Physical review. E, Statistical, nonlinear, and soft matter physics · 2006Article
- Maintenance of stimulus-secretion coupling and single beta-cell function in cryopreserved-thawed human islets of Langerhans.Pflugers Archiv : European journal of physiology · 2005Article
- alpha-Latrotoxin increases spontaneous and depolarization-evoked exocytosis from pancreatic islet beta-cells.The Journal of physiology · 2005Article
- Determination of cell capacitance using the exact empirical solution of partial differential Y/partial differential Cm and its phase angle.Biophysical journal · 2004Article
- A large pool of releasable vesicles in a cortical glutamatergic synapse.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Increased islet apoptosis in Pdx1+/- mice.The Journal of clinical investigation · 2003Article
- Robust, high-resolution, whole cell patch-clamp capacitance measurements using square wave stimulation.Biophysical journal · 2001Article
- The noise of membrane capacitance measurements in the whole-cell recording configuration.Biophysical journal · 2000Article
- Resolution of patch capacitance recordings and of fusion pore conductances in small vesicles.Biophysical journal · 2000Article
- System for dynamic measurements of membrane capacitance in intact epithelial monolayers.Biophysical journal · 1998Article
- alpha-Latrotoxin alters spontaneous and depolarization-evoked quantal release from rat adrenal chromaffin cells: evidence for multiple modes of action.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Fusion pore conductance: experimental approaches and theoretical algorithms.Biophysical journal · 1998Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
We present an optimized solution to the problem of membrane impedance estimation when a patch-clamped cell is stimulated by a dual-frequency, sinusoidal excitation. The complete data set of raw whole-cell current samples is typically reduced, via digital lock-in detection, to measurements of the complex cell model admittance at the two stimulus frequencies. We describe a statistical model of both data sets and demonstrate that the admittance data adequately represent the essential features obtained from the raw data. The parameter estimates obtained by a nonlinear weighted least-squares solution (NWLS), which under normal recording conditions is equivalent to the maximum likelihood solution, essentially obtain the theoretical lower bound on variance established by the Cramér-Rao bound. Our software implementation of the NWLS solution produces estimates of the cell model parameters that are less noisy than other dual-frequency systems. Our system can be used 1) to measure slow changes in membrane capacitance-in the face of large, slow changes in membrane resistance, 2) to detect with confidence capacitance changes expected from the exocytosis of moderate-sized dense core granules, and 3) to reduce the cross-talk between transient changes in membrane conductance and membrane capacitance.
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