ArticleThe Journal of physiology1997
Effect of metabolic inhibition on intracellular Ca2+, phosphorylation of myosin regulatory light chain and force in rat smooth muscle.
Article in The Journal of physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.
- Cyanide Beyond Toxicity: A Systematic Review of Its Effects on Vascular Function.Basic & clinical pharmacology & toxicology · 2025Pooled it
- Modeling and experimental approaches for elucidating multi-scale uterine smooth muscle electro- and mechano-physiology: A review.Frontiers in physiology · 2022Review
- Analysis of decay kinetics of the cytosolic calcium transient induced by oxytocin in rat myometrium smooth muscle cells.Journal of muscle research and cell motility · 2021Article
- Association of rat thoracic aorta dilatation by astragaloside IV with the generation of endothelium-derived hyperpolarizing factors and nitric oxide, and the blockade of CaBiomedical reports · 2016Article
- Physiological increases in lactate inhibit intracellular calcium transients, acidify myocytes and decrease force in term pregnant rat myometrium.The Journal of physiology · 2015Article
- Hypoxia-induced force increase (HIFI) is a novel mechanism underlying the strengthening of labor contractions, produced by hypoxic stresses.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Store-operated Ca²⁺ entry and depolarization explain the anomalous behaviour of myometrial SR: effects of SERCA inhibition on electrical activity, Ca²⁺ and force.Cell calcium · 2014Article
- A comparison of the contractile properties of myometrium from singleton and twin pregnancies.PloS one · 2013Article
- What do we know about what happens to myometrial function as women age?Journal of muscle research and cell motility · 2012Article
- Mitochondrial regulation of cytosolic Ca²⁺ signals in smooth muscle.Pflugers Archiv : European journal of physiology · 2012Review
- Diabetes is associated with impairment of uterine contractility and high Caesarean section rate.Diabetologia · 2012Article
- Poor spontaneous and oxytocin-stimulated contractility in human myometrium from postdates pregnancies.PloS one · 2012Article
- Article
- Regulation of the uterine contractile apparatus and cytoskeleton.Seminars in cell & developmental biology · 2007Review
- Depletion of membrane cholesterol eliminates the Ca2+-activated component of outward potassium current and decreases membrane capacitance in rat uterine myocytes.The Journal of physiology · 2007Article
- Gestational-dependent changes in the expression of signal transduction and contractile filament-associated proteins in mouse myometrium.Journal of the Society for Gynecologic Investigation · 2005Article
- HSP20 phosphorylation and interstitial metabolites in hypoxia-induced dilation of swine coronary arteries.Acta physiologica Scandinavica · 2005Article
- Mitochondrial modulation of Ca2+ sparks and transient KCa currents in smooth muscle cells of rat cerebral arteries.The Journal of physiology · 2004Article
- The role of the sarcoplasmic reticulum in neonatal uterine smooth muscle: enhanced role compared to adult rat.The Journal of physiology · 2002Article
- In vivo pH and metabolite changes during a single contraction in rat uterine smooth muscle.The Journal of physiology · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
1. The effect of the inhibition of oxidative phosphorylation on intracellular calcium concentration ([Ca2+]i), phosphorylation of the 20 kDa regulatory light chain of myosin (MLC20) and contractility was investigated in isolated longitudinal smooth muscle from rat uteri. 2. Cyanide (2 mM) application to normally polarized preparations resulted in an elevation of basal [Ca2+]i but an inhibition of [Ca2+]i transients and the accompanying contractions. 3. Depolarization with high-K+ solution (40 mM KCI) resulted in elevation of [Ca2+]i and maintained force production. Phosphorylation of MLC20 was transiently increased followed by a steady-state augmentation above resting levels. 4. Carbachol (100 microM) produced a transient elevation of [Ca2+]i and force of depolarized tissues followed by a steady-state augmentation of both parameters. PGF2 alpha (1 microM) did not significantly potentiate [Ca2+]i or force in depolarized preparations. Both carbachol and PGF2 alpha potentiated phosphorylation of MLC20 in depolarized tissues. 5. Addition of cyanide to depolarized preparations, in the presence or absence of carbachol or PGF2 alpha, resulted in significant attenuation of force under each condition. The magnitude and normalized rates of force inhibition by cyanide were not significantly different for each stimulus condition. MLC20 phosphorylation levels were unaltered by cyanide treatment. However, cyanide increased the maintained level of [Ca2+]i under each experimental protocol. 6. It is concluded that the inhibition of oxidative phosphorylation with cyanide results in dissociation of both the [Ca2+]i-force and MLC20 phosphorylation-force relationships in rat uterine smooth muscle.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.