Evidence map›Paper›PMID 9080371›Full record

ArticleThe Journal of physiology1997

Alpha 1-adrenoceptor activation of a non-selective cation current in rabbit portal vein by 1,2-diacyl-sn-glycerol.

R M Helliwell, W A Large

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.

0numbers the graph read from it
0cells of the map it votes in
51citing papers in PubMed
1.3field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

51 citing papers in PubMed, 100 citations in OpenAlex.

  1. Review
  2. Review
  3. Calcium signals that determine vascular resistance.Wiley interdisciplinary reviews. Systems biology and medicine · 2019
    Review
  4. Review
  5. Article
  6. Article
  7. Calcium Channels in Vascular Smooth Muscle.Advances in pharmacology (San Diego, Calif.) · 2017
    Review
  8. Article
  9. Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Store-operated calcium entry in vascular smooth muscle.British journal of pharmacology · 2008
    Review
  20. TRP channels in hypertension.Biochimica et biophysica acta · 2007
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

R M HelliwellDepartment of Pharmacology and Clinical Pharmacology, St George's Hospital Medical School, London, UK. r.helliwell@sghms.ac.uk
W A Large
St George's Hospital · GB

Funding

Wellcome Trust
6 · The paper itself

Abstract

1. The transduction mechanisms involved in the activation and modulation of the noradrenaline-activated cation current (Icat) were investigated with whole-cell patch clamp techniques in rabbit portal vein smooth muscle cells. 2. Intracellular application of guanosine 5-O-(3-thiotriphosphate) (GTP gamma S, 500 microM) evoked a 'noisy' inward current at -50 mV with a similar current-voltage relationship and reversal potential to the current evoked by bath application of noradrenaline (100 microM). Guanosine 5-O-(2-thiodiphosphate) (GDP beta S, 1 mM) markedly inhibited noradrenaline-activated Icat. 3. The phospholipase C (PLC) inhibitor U73122 inhibited the amplitude of the noradrenaline-activated Icat in a concentration- and time-dependent manner and the IC50 was about 180 nM. U73122 had similar effects on the cation current evoked by GTP gamma S. 4. Intracellular application of myo-inositol 1,4,5-trisphosphate (IP3, 100 microM) from the patch pipette did not activate any membrane current in cells where intracellular calcium concentration ([Ca2+]i) was buffered to 14 nM, but subsequent addition of noradrenaline evoked Icat. 5. Bath application of the 1,2-diacyl-sn-glycerol (DAG) analogue 1-oleoyl-2-acetyl-sn-glycerol (OAG, 10 microM) activated Icat, whereas the phorbol ester phorbol 12,13-dibutyrate (PDBu, 0.1-5 microM) failed to activate Icat, in every cell examined. Icat activated by OAG after bath application of PDBu was not significantly different from OAG-activated Icat in the absence of PDBu. The DAG lipase inhibitor RHC80267 (10 microM) activated Icat in some cells, whereas the DAG kinase inhibitor R59949 (10 microM) never activated Icat. 6. Bath application of the protein kinase C inhibitor chelerythrine (1-10 microM) had no effect on either OAG-or noradrenaline-activated Icat. 7. It is concluded that noradrenaline activates Icat via a G-protein coupled to PLC and that the resulting DAG product plays a central role in the activation of cation channels via a protein kinase C-independent mechanism.

Indexed as

AlkaloidsAnimalsBenzophenanthridinesCarcinogensCyclohexanonesDiglyceridesEnzyme InhibitorsEstrenesGuanosine 5'-O-(3-Thiotriphosphate)Guanosine DiphosphateInositol 1,4,5-TrisphosphateLipoprotein LipaseNorepinephrinePhenanthridinesPhorbol 12,13-DibutyratePhosphodiesterase Inhibitors1,2-diacylglycerol1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione1,6-bis(cyclohexyloximinocarbonyl)hexaneAlkaloidsBenzophenanthridinesCarcinogenschelerythrineCyclohexanonesDiglyceridesEnzyme InhibitorsEstrenesguanosine 5'-O-(2-thiodiphosphate)Guanosine 5'-O-(3-Thiotriphosphate)Guanosine DiphosphateInositol 1,4,5-TrisphosphateLipoprotein LipaseNorepinephrinePhenanthridinesPhorbol 12,13-DibutyratePhosphodiesterase InhibitorsPyrrolidinonesReceptors, Adrenergic, alpha-1ThionucleotidesType C Phospholipases

Identifiers

PMID9080371
PMCPMC1159316
OpenAlexW2033023357

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.