ArticleThe Journal of physiology1997
Imaging the hierarchical Ca2+ signalling system in HeLa cells.
Article in The Journal of physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers.
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Who cites it
109 citing papers in PubMed, 273 citations in OpenAlex.
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- Endoplasmic Reticulum Calcium Signaling in Hippocampal Neurons.Biomolecules · 2024Review
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- Three-Dimensional Model of Sub-Plasmalemmal CaFrontiers in molecular biosciences · 2022Article
- Calcium Signaling Regulated by Cellular Membrane Systems and Calcium Homeostasis Perturbed in Alzheimer's Disease.Frontiers in cell and developmental biology · 2022Review
- Termination of CaCell calcium · 2021Article
- Stimulation of Local Cytosolic Calcium Release by Photothermal Heating for Studying Intra- and Intercellular Calcium Waves.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- Spatio-temporal feature learning with reservoir computing for T-cell segmentation in live-cell [Formula: see text] fluorescence microscopy.Scientific reports · 2021Article
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- Direct interaction of metastasis-inducing S100P protein with tubulin causes enhanced cell migration without changes in cell adhesion.The Biochemical journal · 2020Article
- NLRP3 inflammasome activation by Foot-and-mouth disease virus infection mainly induced by viral RNA and non-structural protein 2B.RNA biology · 2020Article
- Fundamentals of Cellular Calcium Signaling: A Primer.Cold Spring Harbor perspectives in biology · 2020Review
- Elementary calcium signaling in arterial smooth muscle.Channels (Austin, Tex.) · 2019Review
- Spatial-temporal patterning of CaSeminars in cell & developmental biology · 2019Review
49 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Confocal microscopy was used to investigate hormone-induced subcellular Ca2+ release signals from the endoplasmic reticulum (ER) in a prototype non-excitable cell line (HeLa cells). 2. Histamine application evoked two types of elementary Ca2+ signals: (i) Ca2+ blips arising from single ER Ca2+ release channels (amplitude, 30 nM; lateral spreading, 1.3 microns); (ii) Ca2+ puffs resulting from the concerted activation of several Ca2+ blips (amplitude, 170 nM; spreading, 4 microns). 3. Ca2+ waves in the HeLa cells arose from a variable number of initiation sites, but for individual cells, the number and subcellular location of the initiation sites were constant. The kinetics and amplitude of global Ca2+ signals were directly proportional to the number of initiation sites recruited. 4. Reduction of the feedback inherent in intracellular Ca2+ release caused saltatoric Ca2+ waves, revealing the two principal steps underlying wave propagation: diffusion and regeneration. Threshold stimulation evoked abortive Ca2+ waves, caused by the limited recruitment of Ca2+ puffs. 5. The hierarchy of Ca2+ signalling events, from fundamental levels (blips) to intermediate levels (puffs) to Ca2+ waves, is a prototype for Ca2+ signal transduction for non-excitable cells, and is also analogous to the Ca2+ quarks, Ca2+ sparks and Ca2+ waves in cardiac muscle cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.