ArticleJournal of bacteriology1997
Mutagenesis of Burkholderia pseudomallei with Tn5-OT182: isolation of motility mutants and molecular characterization of the flagellin structural gene.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 124 papers.
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Who cites it
124 citing papers in PubMed, 206 citations in OpenAlex.
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- Burkholderia pseudomallei JW270 Is Lethal in the Madagascar Hissing Cockroach Infection Model and Can Be Utilized at Biosafety Level 2 to Identify Putative Virulence Factors.Infection and immunity · 2022Article
- The NarX-NarL two-component system regulates biofilm formation, natural product biosynthesis, and host-associated survival in Burkholderia pseudomallei.Scientific reports · 2022Article
- The Burkholderia pseudomalleiMicrobiology spectrum · 2021Article
- Functional redundancy of Burkholderia pseudomallei phospholipase C enzymes and their role in virulence.Scientific reports · 2020Article
- An Evolutionary Arms Race BetweenFrontiers in microbiology · 2020Review
- GC-072: A Novel Therapeutic Candidate for Oral Treatment of Melioidosis and Infections Caused by Select Biothreat Pathogens.Antimicrobial agents and chemotherapy · 2019Article
- Role of Toll-Like Receptor 5 (TLR5) in Experimental Melioidosis.Infection and immunity · 2019Article
- Bacteriophage-associated genes responsible for the widely divergent phenotypes of variants of Burkholderia pseudomallei strain MSHR5848.Journal of medical microbiology · 2019Article
- The heritable natural competency trait of Burkholderia pseudomallei in other Burkholderia species through comE and crp.Scientific reports · 2018Article
- Melioidosis.Nature reviews. Disease primers · 2018Review
- Antibodies againstInfection and immunity · 2017Article
- Thermoregulation of Biofilm Formation in Burkholderia pseudomallei Is Disrupted by Mutation of a Putative Diguanylate Cyclase.Journal of bacteriology · 2017Article
- Nitrate Sensing and Metabolism Inhibit Biofilm Formation in the Opportunistic PathogenFrontiers in microbiology · 2017Article
- Transient In Vivo Resistance Mechanisms of Burkholderia pseudomallei to Ceftazidime and Molecular Markers for Monitoring Treatment Response.PLoS neglected tropical diseases · 2017Article
- Immune Modulation as an Effective Adjunct Post-exposure Therapeutic for B. pseudomallei.PLoS neglected tropical diseases · 2016Article
- Versatile nourseothricin and streptomycin/spectinomycin resistance gene cassettes and their use in chromosome integration vectors.Journal of microbiological methods · 2016Article
64 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Burkholderia pseudomallei is a human and animal pathogen in tropical regions, especially Southeast Asia and northern Australia. Currently little is known about the genetics and molecular biology of this organism. In this report, we describe the mutagenesis of B. pseudomallei with the transposon Tn5-OT182. B. pseudomallei 1026b transposon mutants were obtained at a frequency of 4.6 x 10(-4) per initial donor cell, and the transposon inserted randomly into the chromosome. We used Tn5-OT182 to identify the flagellin structural gene, fliC. We screened 3,500 transposon mutants and identified 28 motility mutants. Tn5-OT182 integrated into 19 unique genetic loci encoding proteins with homology to Escherichia coli and Salmonella typhimurium flagellar and chemotaxis proteins. Two mutants, MM35 and MM36, contained Tn5-OT182 integrations in fliC. We cloned and sequenced fliC and used it to complement MM35 and MM36 in trans. The fliC transcriptional start site and a sigmaF-like promoter were identified by primer extension analysis. We observed a significant difference in the expression of two distinct fliC-lacZ transcriptional fusions during bacterial growth, suggesting the presence of a latent intragenic transcriptional terminator in fliC. There was no significant difference in the virulence of 1026b compared to that of MM36 in diabetic rats or Syrian hamsters, suggesting that flagella and/or motility are probably not virulence determinants in these animal models of B. pseudomallei infection. A phylogenetic analysis based on the flagellins from a variety of bacterial species supported the recent transfer of B. pseudomallei from the genus Pseudomonas to Burkholderia.
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