ArticleJournal of virological methods1997
Rapid diagnosis of avian infectious bronchitis virus by the polymerase chain reaction.
Article in Journal of virological methods, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 31 citations in OpenAlex.
- TaqMan quantitative real-time PCR for detecting Avipoxvirus DNA in various sample types from hummingbirds.PloS one · 2020Article
- S1 gene sequence analysis of new variant isolates of avian infectious bronchitis virus in Tunisia.Veterinary medicine (Auckland, N.Z.) · 2012Article
- Effects of cold storage on detection of avian infectious bronchitis virus in chicken carcasses and local antibodies in tracheal washes.Journal of virological methods · 2005Article
- Development of a Western blot assay for detection of antibodies against coronavirus causing severe acute respiratory syndrome.Clinical and diagnostic laboratory immunology · 2004Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A simple, sensitive and specific polymerase chain reaction (PCR) procedure was developed in order to detect infectious bronchitis virus (IBV) directly in tissue samples. Viral RNA was extracted from allantoic fluids and cell cultures infected experimentally with different strains of IBV and from tissues of naturally infected birds. Viral RNA was then amplified and identified by a nested RT-PCR assay using two sets of primers flanking a well-conserved region of the nucleocapsid gene. The selected IBV nucleocapsid sequence was detected successfully by simple direct electrophoresis of amplified material.
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