ArticleGenetics1997
Transposon-disruption of a maize nuclear gene, tha1, encoding a chloroplast SecA homologue: in vivo role of cp-SecA in thylakoid protein targeting.
Article in Genetics, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 78 citations in OpenAlex.
- STIC2 selectively binds ribosome-nascent chain complexes in the cotranslational sorting of Arabidopsis thylakoid proteins.The EMBO journal · 2024Article
- Biochemical and proteomic insights revealed selenium priming induced phosphorus stress tolerance in common bean (Phaseolus vulgaris L.).Molecular biology reports · 2023Article
- Correlated retrograde and developmental regulons implicate multiple retrograde signals as coordinators of chloroplast development in maize.The Plant cell · 2022Article
- Co-Translational Protein Folding and Sorting in Chloroplasts.Plants (Basel, Switzerland) · 2020Review
- Chloroplast Translation: Structural and Functional Organization, Operational Control, and Regulation.The Plant cell · 2018Review
- Genome-wide analysis of thylakoid-bound ribosomes in maize reveals principles of cotranslational targeting to the thylakoid membrane.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Maize RNA polymerase IV defines trans-generational epigenetic variation.The Plant cell · 2013Article
- A short PPR protein required for the splicing of specific group II introns in angiosperm chloroplasts.RNA (New York, N.Y.) · 2012Article
- Plastids contain a second sec translocase system with essential functions.Plant physiology · 2011Article
- Localization and integration of thylakoid protein translocase subunit cpTatC.The Plant journal : for cell and molecular biology · 2009Article
- A member of the Whirly family is a multifunctional RNA- and DNA-binding protein that is essential for chloroplast biogenesis.Nucleic acids research · 2008Article
- Multiple trans-sensing interactions affect meiotically heritable epigenetic states at the maize pl1 locus.Genetics · 2007Article
- Chloroplast SecA and Escherichia coli SecA have distinct lipid and signal peptide preferences.Journal of bacteriology · 2007Article
- Loss or retention of chloroplast DNA in maize seedlings is affected by both light and genotype.Planta · 2006Article
- Article
- CRS1 is a novel group II intron splicing factor that was derived from a domain of ancient origin.RNA (New York, N.Y.) · 2001Article
- Leaf-specific upregulation of chloroplast translocon genes by a CCT motif-containing protein, CIA 2.The Plant cell · 2001Article
- Recruitment of a peptidyl-tRNA hydrolase as a facilitator of group II intron splicing in chloroplasts.The EMBO journal · 2001Article
- Article
- The maize tha4 gene functions in sec-independent protein transport in chloroplasts and is related to hcf106, tatA, and tatB.The Journal of cell biology · 1999Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A nuclear mutant of maize, tha1, which exhibited defects in the translocation of proteins across the thylakoid membrane, was described previously. A transposon insertion at the tha1 locus facilitated the cloning of portions of the tha1 gene. Strong sequence similarity with secA genes from bacteria, pea and spinach indicates that tha1 encodes a SecA homologue (cp-SecA). The tha1-ref allele is either null or nearly so, in that tha1 mRNA is undetectable in mutant leaves and cp-SecA accumulation is reduced > or = 40-fold. These results, in conjunction with the mutant phenotype described previously, demonstrate that cp-SecA functions in vivo to facilitate the translocation of OEC33, PSI-F and plastocyanin but does not function in the translocation of OEC23 and OEC16. Our results confirm predictions for cp-SecA function made from the results of in vitro experiments and establish several new functions for cp-SecA, including roles in the targeting of a chloroplast-encoded protein, cytochrome f, and in protein targeting in the etioplast, a nonphotosynthetic plastid type. Our finding that the accumulation of properly targeted plastocyanin and cytochrome f in tha1-ref thylakoid membranes is reduced only a few-fold despite the near or complete absence of cp-SecA suggests that cp-SecA facilitates but is not essential in vivo for their translocation across the membrane.
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