ArticleGenetics1997
Mutations in the Saccharomyces cerevisiae type 2A protein phosphatase catalytic subunit reveal roles in cell wall integrity, actin cytoskeleton organization and mitosis.
Article in Genetics, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 95 citations in OpenAlex.
- Casein kinase 1 controls components of a TORC2 signaling network in budding yeast.Journal of cell science · 2024Article
- Casein kinase 1 controls components of a TORC2 signaling network in budding yeast.bioRxiv : the preprint server for biology · 2024Article
- Cdc42-Specific GTPase-Activating Protein Rga1 Squelches Crosstalk between the High-Osmolarity Glycerol (HOG) and Mating Pheromone Response MAPK Pathways.Biomolecules · 2021Article
- Article
- Orchestrated Action of PP2A Antagonizes Atg13 Phosphorylation and Promotes Autophagy after the Inactivation of TORC1.PloS one · 2016Article
- Ppg1, a PP2A-type protein phosphatase, controls filament extension and virulence in Candida albicans.Eukaryotic cell · 2014Article
- Cell morphogenesis proteins are translationally controlled through UTRs by the Ndr/LATS target Ssd1.PloS one · 2014Article
- Repression of class I transcription by cadmium is mediated by the protein phosphatase 2A.Nucleic acids research · 2013Article
- PP2A(Cdc55) regulates G1 cyclin stability.Cell cycle (Georgetown, Tex.) · 2013Article
- Dual Regulation of the mitotic exit network (MEN) by PP2A-Cdc55 phosphatase.PLoS genetics · 2013Article
- Spatial regulation of Cdc55-PP2A by Zds1/Zds2 controls mitotic entry and mitotic exit in budding yeast.The Journal of cell biology · 2011Article
- The yeast Cbk1 kinase regulates mRNA localization via the mRNA-binding protein Ssd1.The Journal of cell biology · 2011Article
- The Zds proteins control entry into mitosis and target protein phosphatase 2A to the Cdc25 phosphatase.Molecular biology of the cell · 2011Article
- Cbk1 regulation of the RNA-binding protein Ssd1 integrates cell fate with translational control.Current biology : CB · 2009Article
- H2O2 activates the nuclear localization of Msn2 and Maf1 through thioredoxins in Saccharomyces cerevisiae.Eukaryotic cell · 2009Article
- The protein phosphatase 2A functions in the spindle position checkpoint by regulating the checkpoint kinase Kin4.Genes & development · 2009Article
- Tap42-associated protein phosphatase type 2A negatively regulates induction of autophagy.Autophagy · 2009Article
- Separase cooperates with Zds1 and Zds2 to activate Cdc14 phosphatase in early anaphase.The Journal of cell biology · 2008Article
- Cdc55p-mediated E4orf4 growth inhibition in Saccharomyces cerevisiae is mediated only in part via the catalytic subunit of protein phosphatase 2A.Journal of virology · 2008Article
- Regulation of Mih1/Cdc25 by protein phosphatase 2A and casein kinase 1.The Journal of cell biology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Temperature-sensitive mutations were generated in the Saccharomyces cerevisiae PPH22 gene that, together with its homologue PPH21, encode the catalytic subunit of type 2A protein phosphatase (PP2A). At the restrictive temperature (37 degrees), cells dependent solely on pph22 alleles for PP2A function displayed a rapid arrest of proliferation. Ts pph22 mutant cells underwent lysis at 37 degrees, showing an accompanying viability loss that was suppressed by inclusion of 1 M sorbitol in the growth medium. Ts pph22 mutant cells also displayed defects in bud morphogenesis and polarization of the cortical actin cytoskeleton at 37 degrees. PP2A is therefore required for maintenance of cell integrity and polarized growth. On transfer from 24 degrees to 37 degrees, Ts- pph22 mutant cells accumulated a 2N DNA content indicating a cell cycle block before completion of mitosis. However, during prolonged incubation at 37 degrees, many Ts- pph22 mutant cells progressed through an aberrant nuclear division and accumulated multiple nuclei. Ts- pph22 mutant cells also accumulated aberrant microtubule structures at 37 degrees, while under semi-permissive conditions they were sensitive to the microtubule-destabilizing agent benomyl, suggesting that PP2A is required for normal microtubule function. Remarkably, the multiple defects of Ts- pph22 mutant cells were suppressed by a viable allele (SSD1-v1) of the polymorphic SSD1 gene.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.