ArticleMammalian genome : official journal of the International Mammalian Genome Society1997
Construction of a swine YAC library allowing an efficient recovery of unique and centromeric repeated sequences.
Article in Mammalian genome : official journal of the International Mammalian Genome Society, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 40 citations in OpenAlex.
- Classical, Molecular, and Genomic Cytogenetics of the Pig, a Clinical Perspective.Animals : an open access journal from MDPI · 2021Review
- Nuclear architecture of resting and LPS-stimulated porcine neutrophils by 3D FISH.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009Article
- Interspecific transfer of mammalian artificial chromosomes between farm animals.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009Article
- Genetic resources, genome mapping and evolutionary genomics of the pig (Sus scrofa).International journal of biological sciences · 2007Review
- Characterization of reciprocal translocations in pigs using dual-colour chromosome painting and primed in situ DNA labelling.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1998Article
- Construction and extensive characterization of a goat bacterial artificial chromosome library with threefold genome coverage.Mammalian genome : official journal of the International Mammalian Genome Society · 1998Article
- Localization of the beta 2-microglobulin gene to pig chromosome 1q17.Mammalian genome : official journal of the International Mammalian Genome Society · 1997Article
- Mapping of the melatonin receptor 1a (MTNR1A) gene in pigs, sheep, and cattle.Mammalian genome : official journal of the International Mammalian Genome Society · 1997Article
- Localization of beta-galactoside alpha2,6-sialyltransferase gene on pig chromosome 13q4.1.Mammalian genome : official journal of the International Mammalian Genome Society · 1997Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A swine DNA genomic library was constructed in yeast artificial chromosome (YAC) using the pYAC4 vector and the AB1380 strain. The DNA prepared from two Large White males was partially digested with EcoRI and size selected after both digestion and ligation. The YAC library contained 33792 arrayed clones with an average size of 280 kb as estimated by analysis of 2% of the clones, thus representing a threefold coverage of the swine haploid genome. The library was organized in pools to facilitate the PCR screening. The complexity of the library was tested both for unique and centromeric repeated sequences. In all, 20 out of 22 primer sets allowed the characterization of one to six clones containing specific unique sequences. These sequences are known to be on Chromosomes (Chrs) 1, 2, 5, 6, 7, 8, 13, 14, 15, 17, and X. Eight additional clones carrying centromeric repeat units were also isolated with a single primer set. The sequencing of 37 distinct repeat units of about 340 bp subcloned from these eight YACs revealed high sequence diversity indicating the existence of numerous centromeric repeat unit subfamilies in swine. Furthermore, the analysis of the restriction patterns with selected enzymes suggested a higher order organization of the repeat units. According to preliminary FISH experiments on a small number of randomly chosen YACs and YACs carrying specific sequences, the chimerism appeared to be low. In addition, primed in situ labeling experiments favored the idea that the YACs with centromeric repeat sequences were derived from a subset of metacentric and submetacentric chromosomes.
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