ArticleThe Biochemical journal1997
Regulation of the rat cardiac troponin I gene by the transcription factor GATA-4.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 91 citations in OpenAlex.
- Troponin: biology, molecular mechanisms, and multidimensional clinical applications.Molecular biomedicine · 2026Review
- Targeting GATA4 for cardiac repair.IUBMB life · 2020Review
- Article
- MicroRNAs Association in the Cardiac Hypertrophy Secondary to Complex Congenital Heart Disease in Children.Pediatric cardiology · 2017Article
- Mechanisms of favorable effects of Rho kinase inhibition on myocardial remodeling and systolic function after experimental myocardial infarction in the rat.Therapeutic advances in cardiovascular disease · 2016Article
- Article
- Acetaminophen attenuates doxorubicin-induced cardiac fibrosis via osteopontin and GATA4 regulation: reduction of oxidant levels.Journal of cellular physiology · 2013Article
- Lrrc10 is a novel cardiac-specific target gene of Nkx2-5 and GATA4.Journal of molecular and cellular cardiology · 2013Article
- Cell signaling pathways for the regulation of GATA4 transcription factor: Implications for cell growth and apoptosis.Cellular signalling · 2011Article
- Molecular role of GATA binding protein 4 (GATA-4) in hyperglycemia-induced reduction of cardiac contractility.Cardiovascular diabetology · 2011Article
- Real-time reverse transcription-PCR expression profiling of porcine troponin I family in three different types of muscles during development.Molecular biology reports · 2011Article
- Nox2 NADPH oxidase promotes pathologic cardiac remodeling associated with Doxorubicin chemotherapy.Cancer research · 2010Article
- Identification of three novel SNPs and association with carcass traits in porcine TNNI1 and TNNI2.Molecular biology reports · 2010Article
- Anti-Fas gene therapy prevents doxorubicin-induced acute cardiotoxicity through mechanisms independent of apoptosis.The American journal of pathology · 2010Article
- The CO/HO system reverses inhibition of mitochondrial biogenesis and prevents murine doxorubicin cardiomyopathy.The Journal of clinical investigation · 2007Article
- Motif discovery in tissue-specific regulatory sequences using directed information.EURASIP journal on bioinformatics & systems biology · 2007Article
- Identification of rat lung--prominent genes by a parallel DNA microarray hybridization.BMC genomics · 2006Article
- The slow skeletal muscle troponin T gene is expressed in developing and diseased human heart.Molecular and cellular biochemistry · 2004Article
- Structure and regulation of human troponin genes.Molecular and cellular biochemistry · 2004Article
- Transcription of Drosophila troponin I gene is regulated by two conserved, functionally identical, synergistic elements.Molecular biology of the cell · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Troponin I is a thin-filament contractile protein expressed in striated muscle. There are three known troponin I genes which are expressed in a muscle-fibre-type-specific manner in mature animals. Although the slow skeletal troponin I isoform is expressed in fetal and neonatal heart, the cardiac isoform is restricted in its expression to the myocardium at all developmental stages. To study the regulation of this cardiac-specific and developmentally regulated gene in vitro, the rat cardiac troponin I gene was cloned. Transient transfection assays were performed with troponin I-luciferase fusion plasmids to characterize the regulatory regions of the gene. Proximal regions of the upstream sequence were sufficient to support high levels of expression of the reporter gene in cardiocytes and relatively low levels in other cell types. The highest luciferase activity in the cardiocytes was noted with a plasmid that included the region spanning -896 to +45 of the troponin I genomic sequence. Co-transfection of GATA-4, a recently identified cardiac transcription factor, with troponin I-luciferase constructs permitted high levels of luciferase expression in non-cardiac cells. Electrophoretic mobility-shift assays demonstrated specific binding of GATA-4 to oligonucleotides representative of multiple sites of the troponin I sequence. Mutation of a proximal GATA-4 DNA-binding site decreased transcriptional activation in transfected cardiocytes. These results indicate that the proximal cardiac troponin I sequence is sufficient to support high levels of cardiac-specific gene expression and that the GATA-4 transcription factor regulates troponin I-luciferase expression in vitro.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.