ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1997
Extracellular ATP activates calcium signaling, ion, and fluid transport in retinal pigment epithelium.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed, 127 citations in OpenAlex.
- Review of the clinical electrooculogram - Part 1: Mechanism of the Light-Rise.Documenta ophthalmologica. Advances in ophthalmology · 2026Review
- A biologically validated mathematical model for decoding epithelial apical, basolateral, and paracellular electrical properties.American journal of physiology. Cell physiology · 2023Article
- Retinal TRP channels: Cell-type-specific regulators of retinal homeostasis and multimodal integration.Progress in retinal and eye research · 2023Review
- Cell maturation influences the ability of hESC-RPE to tolerate cellular stress.Stem cell research & therapy · 2022Article
- Culture surface protein coatings affect the barrier properties and calcium signalling of hESC-RPE.Scientific reports · 2021Article
- Mechanisms of Ion Transport Across the Mouse Retinal Pigment Epithelium Measured In Vitro.Investigative ophthalmology & visual science · 2020Article
- Regulatory mechanism for the transmembrane receptor that mediates bidirectional vitamin A transport.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- High-yield, automated intracellular electrophysiology in retinal pigment epithelia.Journal of neuroscience methods · 2019Article
- Purinergic signaling in the retina: From development to disease.Brain research bulletin · 2019Review
- Mouse retinal pigment epithelial cells exhibit a thiocyanate-selective conductance.American journal of physiology. Cell physiology · 2018Article
- ATP activates bestrophin ion channels through direct interaction.Nature communications · 2018Article
- Light-Dependent OCT Structure Changes in Photoreceptor Degenerative rd 10 Mouse Retina.Investigative ophthalmology & visual science · 2018Article
- Primary Cilium-Mediated Retinal Pigment Epithelium Maturation Is Disrupted in Ciliopathy Patient Cells.Cell reports · 2018Article
- Validation of iPS Cell-Derived RPE Tissue in Animal Models.Advances in experimental medicine and biology · 2018Article
- Polarized Human Retinal Pigment Epithelium Exhibits Distinct Surface Proteome on Apical and Basal Plasma Membranes.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Targeting the tight junction protein, zonula occludens-1, with the connexin43 mimetic peptide, αCT1, reduces VEGF-dependent RPE pathophysiology.Journal of molecular medicine (Berlin, Germany) · 2017Article
- Expression of anoctamins in retinal pigment epithelium (RPE).Pflugers Archiv : European journal of physiology · 2016Article
- In Pursuit of Authenticity: Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium for Clinical Applications.Stem cells translational medicine · 2016Article
- Anatomical and Gene Expression Changes in the Retinal Pigmented Epithelium Atrophy 1 (rpea1) Mouse: A Potential Model of Serous Retinal Detachment.Investigative ophthalmology & visual science · 2016Article
- Isothiocyanatostilbenes as novel c-Met inhibitors.Oncotarget · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The presence of receptors for ATP has not been established in any native preparation of retinal neurons or glia. In the present study, we used conventional electrophysiological and [Ca2+]in fluorescence imaging techniques to investigate the effects of ATP added to Ringer's solution perfusing the retinal-facing (apical) membrane of freshly isolated monolayers of bovine retinal pigment epithelium (RPE). ATP (or UTP) produced large, biphasic voltage and resistance changes with a Kd of approximately 5 microM for ATP and approximately 1 microM for UTP. Electrical and pharmacological evidence indicates that the first and second phases of the response are attributable to an increase in basolateral membrane Cl conductance and a decrease in apical membrane K conductance, respectively. The ATP-induced responses were not affected by adenosine, but were reduced by the P2-purinoceptor blocker suramin. ATP also produced a large, transient increase in [Ca2+]in that was blocked by cyclopiazonic acid, an inhibitor of endoplasmic reticulum Ca2+-ATPases. The calcium buffer BAPTA attenuated the voltage effects of ATP. We also found that apical DIDS significantly inhibited the ATP-evoked [Ca2+]in and electrical responses, suggesting that DIDS blocked the purinoceptor. Measurements of fluid movement across the RPE using the capacitance probe technique demonstrated a significant increase in fluid absorption by apical UTP. These data indicate the presence of metabotropic P2Y/P2U-purinoceptors at the RPE apical membrane and implicate extracellular ATP in vivo as a retinal signaling molecule that could help regulate the hydration and chemical composition of the subretinal space.
Indexed as
Identifiers
9065493PMC6573488W2170687030What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.