ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1997
beta43': An enhancer displaying neural-restricted activity is located in the 3'-untranslated exon of the rat nicotinic acetylcholine receptor beta4 gene.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 41 citations in OpenAlex.
- Evidence for Association Between Low Frequency Variants in CHRNA6/CHRNB3 and Antisocial Drug Dependence.Behavior genetics · 2016Article
- Functional characterization of SNPs in CHRNA3/B4 intergenic region associated with drug behaviors.Brain research · 2013Article
- Alternative CHRNB4 3'-UTRs mediate the allelic effects of SNP rs1948 on gene expression.PloS one · 2013Article
- The nicotinic acetylcholine receptor CHRNA5/A3/B4 gene cluster: dual role in nicotine addiction and lung cancer.Progress in neurobiology · 2010Review
- Enhancers of GnRH transcription embedded in an upstream gene use homeodomain proteins to specify hypothalamic expression.Molecular endocrinology (Baltimore, Md.) · 2010Article
- The CHRNA5/A3/B4 gene cluster variability as an important determinant of early alcohol and tobacco initiation in young adults.Biological psychiatry · 2008Article
- Shared long-range regulatory elements coordinate expression of a gene cluster encoding nicotinic receptor heteromeric subtypes.Molecular and cellular biology · 2006Article
- The Ets-transcription factor family in embryonic development: lessons from the amphibian and bird.Oncogene · 2000Review
- Cell type-specific activation of neuronal nicotinic acetylcholine receptor subunit genes by Sox10.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- A highly conserved sequence in the 3'-untranslated region of the drosophila Adh gene plays a functional role in Adh expression.Genetics · 1999Article
- Regulation of ion channel expression in neural cells by hormones and growth factors.Molecular neurobiology · 1998Review
- Neuronal expression of the 5HT3 serotonin receptor gene requires nuclear factor 1 complexes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Members of a neuronal nicotinic acetylcholine receptor subunit gene cluster ordered beta4, alpha3, alpha5 in the vertebrate genome are expressed in highly restricted patterns in the PNS and CNS. Nothing is known, however, about the regulatory elements that control transcription of these genes in selected neuronal cell populations. We report here a novel enhancer, designated beta43', that is positioned in the beta4 3'-untranslated exon. It is composed of two nearly identical 37 bp direct repeats that are separated by 6 bp. Multimerization of the enhancer upstream of the alpha3 minimal promoter results in synergistic activation. Analysis in different cell types, including three neural lines and primary keratinocytes, shows that beta43' is preferentially active in the neural line PC12, which expresses all members of the cluster. Mobility shift assays reveal a cell-type-specific complex, which forms with the first repeat of the enhancer and PC12 extracts. Complexes co-migrating with the PC12 cell complex are not detected with extracts from other lines, which suggests that PC12 cells contain a differentially expressed factor that may be important for the restricted activity of beta43'. The cell-type-specific activity of the beta43' enhancer suggests that it is important for regulating restricted expression patterns of one or more clustered neuronal acetylcholine receptor genes. Its location within the beta4 gene may be a selective pressure for maintaining tight linkage of clustered neuronal nAchR genes.
Indexed as
Identifiers
9065489PMC6573506W2143466882What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.