ArticleThe Plant cell1997
The wheat transcriptional activator SPA: a seed-specific bZIP protein that recognizes the GCN4-like motif in the bifactorial endosperm box of prolamin genes.
Article in The Plant cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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84 citing papers in PubMed, 193 citations in OpenAlex.
- Genetic regulation of major immunogenic protein accumulation in peanut seeds.Functional & integrative genomics · 2026Article
- Genome-Wide Screening for MYB Transcription Factors Involved in Flavonoid Glycoside Biosynthesis inGenes · 2025Article
- Regulation of HvASN1 expression by bZIP transcription factors during barley embryo development and germination.Planta · 2025Article
- The dynamic epigenetic atlas and its effects on instability of starch and seed storage protein quality traits in wheat (Frontiers in plant science · 2025Article
- A systems review of grain proteins in rice and barley: biosynthesis, regulation, and impact on end-use quality.Frontiers in plant science · 2025Review
- Optimized barley phytase gene expression by focused FIND-IT screening for mutations inFrontiers in plant science · 2024Article
- Storage protein activator controls grain protein accumulation in bread wheat in a nitrogen dependent manner.Scientific reports · 2023Article
- Genetic control of grain amino acid composition in a UK soft wheat mapping population.The plant genome · 2023Article
- Wheat bZIPC1 interacts with FT2 and contributes to the regulation of spikelet number per spike.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Efficient proteome-wide identification of transcription factors targeting Glu-1: A case study for functional validation of TaB3-2A1 in wheat.Plant biotechnology journal · 2023Article
- RNA polymerase common subunit ZmRPABC5b is transcriptionally activated by Opaque2 and essential for endosperm development in maize.Nucleic acids research · 2023Article
- The Landscapes of Gluten Regulatory Network in Elite Wheat Cultivars Contrasting in Gluten Strength.International journal of molecular sciences · 2023Article
- Genome-Wide Tissue-Specific Genes Identification for Novel Tissue-Specific Promoters Discovery in Soybean.Genes · 2023Article
- Wheat NAC-A18 regulates grain starch and storage proteins synthesis and affects grain weight.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Wheat DOF transcription factors TaSAD and WPBF regulate glutenin gene expression in cooperation with SPA.PloS one · 2023Article
- Evaluating relationships between seed morphological traits and seed dormancy inFrontiers in plant science · 2023Article
- Divergence of functions and expression patterns of soybean bZIP transcription factors.Frontiers in plant science · 2023Review
- Underlying Biochemical and Molecular Mechanisms for Seed Germination.International journal of molecular sciences · 2022Review
- TheInternational journal of molecular sciences · 2022Article
- Wheat Quality Formation and Its Regulatory Mechanism.Frontiers in plant science · 2022Review
24 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The conserved bifactorial endosperm box found in the promoter of wheat storage protein genes comprises two different cis elements that are thought to be involved in regulating endosperm-specific gene expression. Endosperm nuclear extracts contain binding activities. One is called ESBF-I, which binds to the endosperm motif (EM), and the other is called ESBF-II, which binds to the GCN4-like motif(GLM). Here, we present a functional analysis of the endosperm box of a low-molecular-weight glutenin gene found on the 1D1 chromosome of hexaploid wheat (LMWG-1D1) in transgenic tobacco plants. Our analysis demonstrates the necessity of the EM and GLM for endosperm-specific gene expression and suggests the presence in tobacco of functional counterparts of wheat ESBF-I and ESBF-II. Furthermore, we describe the isolation and characterization of cDNA clones encoding SPA, a seed-specific basic leucine zipper protein from wheat that can activate transcription from the GLMs of the -326-bp LMWG-1D1 promoter in both maize and tobacco leaf protoplasts. This activation is also partially dependent on the presence of functional EMs, suggesting interactions between SPA with ESBF-I-like activities.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.