ArticleThe Journal of physiology1997
alpha 1-adrenoceptor-mediated negative inotropy of adrenaline in rat myocardium.
Article in The Journal of physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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2 citing papers in PubMed, 16 citations in OpenAlex.
- An endogenous sensitizer of β adrenergic receptors and its analogs attenuate the inhibition of β adrenergic receptors by propranolol and atenolol in the rat myocardium.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections · 2014Article
- Physiological antagonism between ventricular beta 1-adrenoceptors and alpha 1-adrenoceptors but no evidence for beta 2- and beta 3-adrenoceptor function in murine heart.British journal of pharmacology · 2002Article
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
1. The effect of alpha- and beta-adrenoceptor stimulation on isotonic contraction was investigated on right ventricular papillary muscles of the rat, stimulated at a rate of 0.5 Hz. 2. Adrenaline (0.5 microM) induced a slight but significant negative inotropic effect: shortening decreased from 0.137 +/- 0.058 to 0.122 +/- 0.059 muscle lengths (mean +/- S.D.; -11%, P < 0.0001) and maximum shortening velocity from 2.9 +/- 1.2 to 2.7 +/- 1.3 muscle lengths s-1 (-7%, P < 0.025). 3. The negative inotropic effect of adrenaline was enhanced after blocking the beta-adrenoceptors with 50 microM atenolol. On the other hand, exposure to adrenaline after blocking the alpha-adrenoceptors with 50 microM phentolamine resulted in an increase in shortening as well as in maximum shortening velocity. 4. Stimulation of the beta-adrenoceptors with 0.5 microM isoprenaline caused marked positive inotropic effects, whereas stimulation of the alpha 1-adrenoceptors with 0.5 microM phenylephrine regularly resulted in a long-lasting decrease in shortening and maximum shortening velocity. 5. 1,2-Dioctanoyl-sn-glycerol (1,2-DOG) and adrenaline induced an activation of protein kinase C (PKC) with translocation of this enzyme from the cytosol to the sarcolemma. 6. Activation of PKC with 10 microM 1,2-DOG and 0.5 microM adrenaline was accompanied by a decrease in shortening and maximum shortening velocity. Inhibition of PKC with 0.1 microM staurosporine abolished the negative inotropic effect of adrenaline. 7. From these results we conclude that a low dose of adrenaline stimulates not only beta-but also alpha-adrenoceptors and that the observed negative inotropic effect of adrenaline is mediated by alpha 1-adrenoceptors, linked to the diacylglycerol-PKC signal transduction pathway.
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