ArticleJournal of virology1997
Replication of flock house virus RNAs from primary transcripts made in cells by RNA polymerase II.
Article in Journal of virology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Parallel ClickSeq and Nanopore sequencing elucidates the rapid evolution of defective-interfering RNAs in Flock House virus.PLoS pathogens · 2017Article
- Review
- Review
- Rapid evolution of virus sequences in intrinsically disordered protein regions.PLoS pathogens · 2014Article
- Orsay virus utilizes ribosomal frameshifting to express a novel protein that is incorporated into virions.Virology · 2014Article
- Viruses and antiviral immunity in Drosophila.Developmental and comparative immunology · 2014Review
- Article
- Transgenerational inheritance of an acquired small RNA-based antiviral response in C. elegans.Cell · 2011Article
- Mitochondrion-enriched anionic phospholipids facilitate flock house virus RNA polymerase membrane association.Journal of virology · 2009Article
- Replication-coupled packaging mechanism in positive-strand RNA viruses: synchronized coexpression of functional multigenome RNA components of an animal and a plant virus in Nicotiana benthamiana cells by agroinfiltration.Journal of virology · 2008Article
- Three-dimensional analysis of a viral RNA replication complex reveals a virus-induced mini-organelle.PLoS biology · 2007Article
- Identification of nonessential regions of the nsp2 replicase protein of porcine reproductive and respiratory syndrome virus strain VR-2332 for replication in cell culture.Journal of virology · 2007Article
- In vivo self-interaction of nodavirus RNA replicase protein a revealed by fluorescence resonance energy transfer.Journal of virology · 2005Article
- Engineered retargeting of viral RNA replication complexes to an alternative intracellular membrane.Journal of virology · 2003Article
- Flock house virus RNA polymerase is a transmembrane protein with amino-terminal sequences sufficient for mitochondrial localization and membrane insertion.Journal of virology · 2002Article
- Flock house virus RNA replicates on outer mitochondrial membranes in Drosophila cells.Journal of virology · 2001Article
- Characterization and construction of functional cDNA clones of Pariacoto virus, the first Alphanodavirus isolated outside Australasia.Journal of virology · 2000Article
- Stable high-level expression of heterologous genes in vitro and in vivo by noncytopathic DNA-based Kunjin virus replicon vectors.Journal of virology · 2000Article
- Induction and maintenance of autonomous flock house virus RNA1 replication.Journal of virology · 1999Article
- Regulated expression of a Sindbis virus replicon by herpesvirus promoters.Journal of virology · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
To develop vector systems that combine high transcription activity with biologically safe delivery vehicles, we have explored the use of RNA replication to amplify mRNAs, by using flock house virus (FHV) as a model system. The FHV RNA replicase is encoded in the larger of the two segments that comprise the viral positive-sense RNA genome. A cDNA copy of this self-replicating RNA was precisely positioned between a promoter site for cellular RNA polymerase II and a cDNA encoding a self-cleaving ribozyme from hepatitis delta virus. Transfection of this plasmid into cultured BHK cells resulted in prolonged, autonomous FHV RNA replication in the cytoplasm and substantial amplification of the RNA replicon. The replicase also amplified RNA transcribed from a second plasmid of similar design that contained a cDNA copy of the other FHV genome segment. These results constitute a significant step toward the harnessing of nodaviral RNA replication as the basis of a versatile vector system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.