ArticleThe Journal of cell biology1997
Rapid plasma membrane anchoring of newly synthesized p59fyn: selective requirement for NH2-terminal myristoylation and palmitoylation at cysteine-3.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 187 citations in OpenAlex.
- Regulation of T cell function by protein S-acylation.Frontiers in physiology · 2022Review
- T cell receptor (TCR) signaling in health and disease.Signal transduction and targeted therapy · 2021Review
- Function of Protein S-Palmitoylation in Immunity and Immune-Related Diseases.Frontiers in immunology · 2021Review
- Optogenetic Control for Investigating Subcellular Localization of Fyn Kinase Activity in Single Live Cells.Journal of molecular biology · 2020Article
- Live-cell monitoring of protein localization to membrane rafts using protein-fragment complementation.Bioscience reports · 2020Article
- Protein palmitoylation and cancer.EMBO reports · 2018Review
- Intrinsic properties and plasma membrane trafficking route of Src family kinase SH4 domains sensitive to retargeting by HIV-1 Nef.The Journal of biological chemistry · 2018Article
- Phosphorylation of SNAP-23 regulates its dynamic membrane association during mast cell exocytosis.Biology open · 2017Article
- Article
- Fyn in Neurodevelopment and Ischemic Brain Injury.Developmental neuroscience · 2015Review
- Heterologous Src homology 4 domains support membrane anchoring and biological activity of HIV-1 Nef.The Journal of biological chemistry · 2014Article
- Fyn tyrosine kinase increases Apolipoprotein E Receptor 2 levels and phosphorylation.PloS one · 2014Article
- Regulation of the trafficking and antiviral activity of IFITM3 by post-translational modifications.Future microbiology · 2014Review
- Tangles, Toxicity, and Tau Secretion in AD - New Approaches to a Vexing Problem.Frontiers in neurology · 2013Review
- The N-terminal region of IFITM3 modulates its antiviral activity by regulating IFITM3 cellular localization.Journal of virology · 2012Article
- ER-bound protein tyrosine phosphatase PTP1B interacts with Src at the plasma membrane/substrate interface.PloS one · 2012Article
- Glia unglued: how signals from the extracellular matrix regulate the development of myelinating glia.Developmental neurobiology · 2011Review
- Myristoylation and membrane binding regulate c-Src stability and kinase activity.Molecular and cellular biology · 2010Article
- Fyn kinase function in lipid utilization: a new upstream regulator of AMPK activity?Archives of physiology and biochemistry · 2009Review
- Palmitoylation-dependent plasma membrane transport but lipid raft-independent signaling by linker for activation of T cells.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The trafficking of Src family proteins after biosynthesis is poorly defined. Here we studied the role of dual fatty acylation with myristate and palmitate in biosynthetic transport of p59fyn. Metabolic labeling of transfected COS or NIH 3T3 cells with [35S]methionine followed by analysis of cytosolic and total membrane fractions showed that Fyn became membrane bound within 5 min after biosynthesis. Newly synthesized Src, however, accumulated in the membranes between 20-60 min. Northern blotting detected Fyn mRNA specifically in soluble polyribosomes and soluble Fyn protein was only detected shortly (1-2 min) after radiolabeling. Use of chimeric Fyn and Src constructs showed that rapid membrane targeting was mediated by the myristoylated NH2-terminal sequence of Fyn and that a cysteine at position 3, but not 6, was essential. Examination of G alpha(o)-, G alpha(s)-, or GAP43-Fyn fusion constructs indicated that rapid membrane anchoring is exclusively conferred by the combination of N-myristoylation plus palmitoylation of cysteine-3. Density gradient analysis colocalized newly synthesized Fyn with plasma membranes. Interestingly, a 10-20-min lag phase was observed between plasma membrane binding and the acquisition of non-ionic detergent insolubility. We propose a model in which synthesis and myristoylation of Fyn occurs on soluble ribosomes, followed by rapid palmitoylation and plasma membrane anchoring, and a slower partitioning into detergent-insoluble membrane subdomains. These results serve to define a novel trafficking pathway for Src family proteins that are regulated by dual fatty acylation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.