ArticleJournal of clinical pathology1997
Use of methyl methacrylate resin for embedding bone marrow trephine biopsy specimens.
Article in Journal of clinical pathology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed, 31 citations in OpenAlex.
- An Improved Immunostaining and Imaging Methodology to Determine Cell and Protein Distributions within the Bone Environment.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2016Article
- Localization of mRNAs and proteins in methyl methacrylate-embedded tissues.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2009Article
- Optimal processing of bone marrow trephine biopsy: the Hammersmith Protocol.Journal of clinical pathology · 2006Review
- How we process trephine biopsy specimens: epoxy resin embedded bone marrow biopsies.Journal of clinical pathology · 2005Article
- Co-localization of multiple antigens and specific DNA. A novel method using methyl methacrylate-embedded semithin serial sections and catalyzed reporter deposition.The American journal of pathology · 2000Article
- Non-isotopic in situ hybridization to detect chick Sox gene mRNA in plastic-embedded tissue.The Histochemical journal · 1997Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTo evaluate the use of methyl methacrylate resin as an embedding medium for undecalcified bone marrow trephine biopsy specimens.
methodsAbout 2500 undecalcified bone marrow trephine biopsy specimens were processed, and embedded in methyl methacrylate resin. Semithin sections (2-3 microns) were stained by routine tinctorial and immunocytochemical staining methods with a wide range of antibodies using a standard streptavidin biotin horseradish peroxidase technique. Different antigen retrieval pretreatments were evaluated.
resultsBone marrow trephine biopsy specimens are embedded routinely in methyl methacrylate at the Haematological Malignancy Diagnostic Service at The Leeds General Infirmary. Over 50 different primary antibodies are in current use; for the majority of these, microwave antigen retrieval or trypsin digestion, or both, is either essential or greatly enhances the results.
conclusionsEmbedding bone marrow trephine biopsy specimens in methyl methacrylate resin retains morphology and permits reliable, high quality immunocytochemistry. This is particularly desirable for the demonstration of neoplastic cells in regenerative marrow after chemotherapy, and in the detection of residual disease after treatment. The use of methyl methacrylate for routine use on bone marrow trephine biopsy specimens is advocated.
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Registered trials
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