ArticleJournal of inherited metabolic disease1995
Immunocytochemical localization of peroxisomal proteins in human liver and kidney.
Article in Journal of inherited metabolic disease, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 32 citations in OpenAlex.
- Phenylbutyrate up-regulates the adrenoleukodystrophy-related gene as a nonclassical peroxisome proliferator.The Journal of cell biology · 2005Article
- Immunohistochemical demonstration of the amphetamine derivatives 3,4-methylenedioxymethamphetamine (MDMA) and 3,4-methylenedioxyamphetamine (MDA) in human post-mortem brain tissues and the pituitary gland.International journal of legal medicine · 2003Article
- Regulation of catalase enzyme activity by cell signaling molecules.Molecular and cellular biochemistry · 2002Article
- A PEX6-defective peroxisomal biogenesis disorder with severe phenotype in an infant, versus mild phenotype resembling Usher syndrome in the affected parents.American journal of human genetics · 2002Article
- Characterization of rat liver malonyl-CoA decarboxylase and the study of its role in regulating fatty acid metabolism.The Biochemical journal · 2000Article
- Extracerebral biopsy in lysosomal and peroxisomal disorders. Ultrastructural findings.Brain pathology (Zurich, Switzerland) · 1998Review
- Contributions of the immunogold technique to investigation of the biology of peroxisomes.Histochemistry and cell biology · 1996Review
- Immunoblot analysis of peroxisomal proteins in liver and fibroblasts from patients.Journal of inherited metabolic disease · 1995Article
- Liver and chorion cytochemistry.Journal of inherited metabolic disease · 1995Article
- Hepatocyte polarity and the peroxisomal compartment: a comparative study.The Histochemical journalArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sample preparation and immunocytochemical methods for investigating the presence and subcellular localization of peroxisomal proteins (catalase, the three beta-oxidation enzymes, alanine : glyoxylate aminotransferase and a peroxisomal membrane protein) in human liver biopsies are described. We present a protocol for immunolabelling on ultrathin and semithin sections from the same tissue block, with protein A-colloidal gold as a reporter system. For this purpose, the tissue is embedded in Unicryl, a hydrophilic acrylic resin that is cured by ultraviolet illumination at 2 degrees C. The limitations and possibilities of the methods are discussed together with methodological problems. Cryostat sections of prefixed material should be used for the visualization by light microscopy of cytoplasmic catalase. It is emphasized that immunolabelling for catalase in formalin-fixed archival liver samples and in liver autopsy tissue (in the latter also for the peroxisomal beta-oxidation enzymes) permits visualization of peroxisomes; this can be helpful in diagnosing an index case retrospectively.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.