ArticleProceedings of the National Academy of Sciences of the United States of America1997
Cytohesin-1, a cytosolic guanine nucleotide-exchange protein for ADP-ribosylation factor.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 147 citations in OpenAlex.
- CYTH4 Facilitates Renal Cell Carcinoma via Enhancing Proliferation and Likely Immune Evasion.Biomolecules · 2026Article
- Physiological and Pathological Roles of the Cytohesin Family in Neurons.International journal of molecular sciences · 2022Review
- Role of curcumin in PLD activation by Arf6-cytohesin1 signaling axis in U46619-stimulated pulmonary artery smooth muscle cells.Molecular and cellular biochemistry · 2018Article
- Rab35, acting through ACAP2 switching off Arf6, negatively regulates oligodendrocyte differentiation and myelination.Molecular biology of the cell · 2014Article
- In vivo expression of the Arf6 Guanine-nucleotide exchange factor cytohesin-1 in mice exhibits enhanced myelin thickness in nerves.Journal of molecular neuroscience : MN · 2013Article
- Salmonella virulence effector SopE and Host GEF ARNO cooperate to recruit and activate WAVE to trigger bacterial invasion.Cell host & microbe · 2012Article
- Role of PDZ proteins in regulating trafficking, signaling, and function of GPCRs: means, motif, and opportunity.Advances in pharmacology (San Diego, Calif.) · 2011Review
- Molecular cloning, sequence and expression analysis of ZmArf2, a maize ADP-ribosylation factor.Molecular biology reports · 2010Article
- FRMD4A regulates epithelial polarity by connecting Arf6 activation with the PAR complex.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Structural basis and mechanism of autoregulation in 3-phosphoinositide-dependent Grp1 family Arf GTPase exchange factors.Molecular cell · 2007Article
- BCL2 translocation defines a unique tumor subset within the germinal center B-cell-like diffuse large B-cell lymphoma.The American journal of pathology · 2004Article
- Phylogenetic analysis of Sec7-domain-containing Arf nucleotide exchangers.Molecular biology of the cell · 2004Article
- The guanine nucleotide exchange factor ARNO mediates the activation of ARF and phospholipase D by insulin.BMC cell biology · 2003Article
- Role for Arf3p in development of polarity, but not endocytosis, in Saccharomyces cerevisiae.Molecular biology of the cell · 2003Article
- Cybr, a cytokine-inducible protein that binds cytohesin-1 and regulates its activity.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- The Phox homology (PX) domain, a new player in phosphoinositide signalling.The Biochemical journal · 2001Review
- Controlling small guanine-nucleotide-exchange factor function through cytoplasmic RNA intramers.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- ARF-GEP(100), a guanine nucleotide-exchange protein for ADP-ribosylation factor 6.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- The TITAN5 gene of Arabidopsis encodes a protein related to the ADP ribosylation factor family of GTP binding proteins.The Plant cell · 2000Article
- Cytohesin-1 regulates beta-2 integrin-mediated adhesion through both ARF-GEF function and interaction with LFA-1.The EMBO journal · 2000Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytohesin-1, a protein abundant in cells of the immune system, has been proposed to be a human homolog of the Saccharomyces cerevisiae Sec7 gene product, which is crucial in protein transport. More recently, the same protein has been reported to be a regulatory factor for the alphaLbeta2 integrin in lymphocytes. Overexpression of human or yeast ADP-ribosylation factor (ARF) genes rescues yeast with Sec7 defects, restoring secretory pathway function. ARFs, 20-kDa guanine nucleotide-binding proteins initially identified by their ability to stimulate cholera toxin ADP-ribosyltransferase activity and now recognized as critical components in intracellular vesicular transport, exist in an inactive cytosolic form with GDP bound (ARF-GDP). Interaction with a guanine nucleotide-exchange protein (GEP) accelerates exchange of GDP for GTP, producing the active ARF-GTP. Both soluble and particulate GEPs have been described. To define better the interaction between ARF and Sec7-related proteins, effects of cytohesin-1, synthesized in Escherichia coli, on ARF activity were evaluated. Cytohesin-1 enhanced binding of 35S-labeled guanosine 5'-[gamma-thio]triphosphate [35S]GTP[gammaS] or [3H]GDP to ARF purified from bovine brain (i.e., it appeared to function as an ARF-GEP). Addition of cytohesin-1 to ARF3 with [35S]GTP[gammaS] bound, accelerated [35S]GTP[gammaS] release to a similar degree in the presence of unlabeled GDP or GTP[gammaS] and to a lesser degree with GDP[betaS]; release was negligible without added nucleotide. Cytohesin-1 also increased ARF1 binding to a Golgi fraction, but its effect was not inhibited by brefeldin A (BFA), a drug that reversibly inhibits Golgi function. In this regard, it differs from a recently reported BFA-sensitive ARF-GEP that contains a Sec7 domain.
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