ArticleProceedings of the National Academy of Sciences of the United States of America1997
Galactose-dependent reversible interaction of Gal3p with Gal80p in the induction pathway of Gal4p-activated genes of Saccharomyces cerevisiae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 107 citations in OpenAlex.
- Mining Genetically Encoded Biosensors from Filamentous Fungi.Journal of fungi (Basel, Switzerland) · 2026Review
- The galactokinase enzyme of yeast senses metabolic flux to stabilize galactose pathway regulation.Nature metabolism · 2025Article
- A unique metabolic gene cluster regulates lactose and galactose metabolism in the yeastApplied and environmental microbiology · 2024Article
- LowTempGAL: a highly responsive low temperature-inducible GAL system in Saccharomyces cerevisiae.Nucleic acids research · 2024Article
- Transcriptional repressor Gal80 recruits corepressor complex Cyc8-Tup1 to structural genes of the Saccharomyces cerevisiae GAL regulon.Current genetics · 2022Article
- Achieving global perfect homeostasis through transporter regulation.PLoS computational biology · 2017Article
- Unexpected functions of lncRNAs in gene regulation.Communicative & integrative biology · 2013Review
- Long noncoding RNAs promote transcriptional poising of inducible genes.PLoS biology · 2013Article
- Self-association of the Gal4 inhibitor protein Gal80 is impaired by Gal3: evidence for a new mechanism in the GAL gene switch.Molecular and cellular biology · 2013Article
- The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation.Genes & development · 2012Article
- Characterizing the memory of the GAL regulatory network in Saccharomyces cerevisiae.Systems and synthetic biology · 2011Article
- Rapid GAL gene switch of Saccharomyces cerevisiae depends on nuclear Gal3, not nucleocytoplasmic trafficking of Gal3 and Gal80.Genetics · 2011Article
- Article
- Extensive in vivo metabolite-protein interactions revealed by large-scale systematic analyses.Cell · 2010Article
- Alterations in the interaction between GAL4 and GAL80 effect regulation of the yeast GAL regulon mediated by the F box protein Dsg1.Current microbiology · 2010Article
- Inducible gene expression: diverse regulatory mechanisms.Nature reviews. Genetics · 2010Review
- Gene activation by dissociation of an inhibitor from a transcriptional activation domain.Molecular and cellular biology · 2009Article
- Serendipity.The Journal of biological chemistry · 2009Article
- Moonlighting proteins in yeasts.Microbiology and molecular biology reviews : MMBR · 2008Review
- Genetic evidence for sites of interaction between the Gal3 and Gal80 proteins of the Saccharomyces cerevisiae GAL gene switch.Genetics · 2008Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When galactose is added to logarithmically growing culture of the yeast Saccharomyces cerevisiae, a set of genes encoding galactose-metabolizing enzymes (GAL genes) starts to be transcribed within a few minutes. This rapid induction involves a serial interplay of Gal3p, Gal80p, and Gal4p. Recent experiments have indicated that a direct interaction between Gal3p and Gal80p plays a pivotal role in an early step of GAL induction. Here we demonstrate that complex of Gal3p and Gal80p, otherwise unstable, is stabilized in the presence of 0.1 mM galactose and 0.5 mM ATP. The requirement for galactose and ATP for stable complex formation is also observed by using highly purified Gal3p and Gal80p from yeast. We further show that thus formed Gal3p/Gal80p complex can easily be dissociated when it is washed with buffer lacking galactose. Finally, we show that mutant proteins encoded by GAL80S or GAL80DE21, which confer galactose-uninducible phenotype, fail to interact with Gal3p. These results strongly suggest that Gal3p functions as the sensor and transducer of galactose signal in the induction pathway of Gal4p-activated genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.