ArticleProceedings of the National Academy of Sciences of the United States of America1997
Gene transfer by guanidinium-cholesterol cationic lipids into airway epithelial cells in vitro and in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 89 citations in OpenAlex.
- Cationic cholesterol derivative efficiently delivers the genes: in silico and in vitro studies.Drug delivery and translational research · 2019Article
- Review
- Nanomedicine in pulmonary delivery.International journal of nanomedicine · 2009Review
- Combined suicide gene therapy for pancreatic peritoneal carcinomatosis using BGTC liposomes.Cancer gene therapy · 2004Article
- Article
- Aerosol gene therapy.Molecular biotechnology · 2003Review
- Novel water insoluble lipoparticulates for gene delivery.Pharmaceutical research · 2002Article
- Delivery systems for pulmonary gene therapy.American journal of respiratory medicine : drugs, devices, and other interventions · 2002Review
- Gene therapy of cystic fibrosis: the glycofection approach.Glycoconjugate journal · 2001Review
- Article
- Mechanism of lipoplex gene delivery in mouse lung: binding and internalization of fluorescent lipid and DNA components.Gene therapy · 2001Article
- Biodistribution and transgene expression with nonviral cationic vector/DNA complexes in the lungs.Gene therapy · 2000Article
- An integrin-targeted non-viral vector for pulmonary gene therapy.Gene therapy · 2000Article
- Mannose receptor-mediated gene transfer into macrophages using novel mannosylated cationic liposomes.Gene therapy · 2000Article
- Structural characteristics of supramolecular assemblies formed by guanidinium-cholesterol reagents for gene transfection.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Synthetic DNA-compacting peptides derived from human sequence enhance cationic lipid-mediated gene transfer in vitro and in vivo.Gene therapy · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthetic vectors represent an attractive alternative approach to viral vectors for gene transfer, in particular into airway epithelial cells for lung-directed gene therapy for cystic fibrosis. Having recently found that guanidinium-cholesterol cationic lipids are efficient reagents for gene transfer into mammalian cell lines in vitro, we have investigated their use for gene delivery into primary airway epithelial cells in vitro and in vivo. The results obtained indicate that the lipid bis(guanidinium)-tren-cholesterol (BGTC) can be used to transfer a reporter gene into primary human airway epithelial cells in culture. Furthermore, liposomes composed of BGTC and dioleoyl phosphatidylethanolamine (DOPE) are efficient for gene delivery to the mouse airway epithelium in vivo. Transfected cells were detected both in the surface epithelium and in submucosal glands. In addition, the transfection efficiency of BGTC/DOPE liposomes in vivo was quantitatively assessed by using the luciferase reporter gene system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.