ArticleThe EMBO journal1997
The role of branchpoint-3' splice site spacing and interaction between intron terminal nucleotides in 3' splice site selection in Saccharomyces cerevisiae.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 78 citations in OpenAlex.
- Splicing of branchpoint-distant exons is promoted by Cactin, Tls1 and the ubiquitin-fold-activated Sde2.Nucleic acids research · 2022Article
- A broad analysis of splicing regulation in yeast using a large library of synthetic introns.PLoS genetics · 2021Article
- Structures of the human spliceosomes before and after release of the ligated exon.Cell research · 2019Article
- Article
- Postcatalytic spliceosome structure reveals mechanism of 3'-splice site selection.Science (New York, N.Y.) · 2017Article
- A birth of bipartite exon by intragenic deletion.Molecular genetics & genomic medicine · 2017Article
- Article
- Spliceosome-mediated decay (SMD) regulates expression of nonintronic genes in budding yeast.Genes & development · 2013Article
- Intronic sequence elements impede exon ligation and trigger a discard pathway that yields functional telomerase RNA in fission yeast.Genes & development · 2013Article
- RNA secondary structure mediates alternative 3'ss selection in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2012Article
- Cotranscriptional spliceosome assembly and splicing are independent of the Prp40p WW domain.RNA (New York, N.Y.) · 2011Article
- Secondary structure is required for 3' splice site recognition in yeast.Nucleic acids research · 2011Article
- A novel spliceosome-mediated trans-splicing can change our view on genome complexity of the divergent eukaryote Giardia intestinalis.Biophysical reviews · 2011Review
- Factors affecting splicing strength of yeast genes.Comparative and functional genomics · 2011Article
- Allele-specific recognition of the 3' splice site of INS intron 1.Human genetics · 2010Article
- Exon sequences at the splice junctions affect splicing fidelity and alternative splicing.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Oriented scanning is the leading mechanism underlying 5' splice site selection in mammals.PLoS genetics · 2006Article
- Exon ligation is proofread by the DExD/H-box ATPase Prp22p.Nature structural & molecular biology · 2006Article
- A mutational analysis of U12-dependent splice site dinucleotides.RNA (New York, N.Y.) · 2005Article
- Biased exon/intron distribution of cryptic and de novo 3' splice sites.Nucleic acids research · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A conserved 3' splice site YAG is essential for the second step of pre-mRNA splicing but no trans-acting factor recognizing this sequence has been found. A direct, non-Watson-Crick interaction between the intron terminal nucleotides was suggested to affect YAG selection. The mechanism of YAG recognition was proposed to involve 5' to 3' scanning originating from the branchpoint or the polypyrimidine tract. We have constructed a yeast intron harbouring two closely spaced 3' splice sites. Preferential selection of a wild-type site over mutant ones indicated that the two sites are competing. For two identical sequences, the proximal site is selected. As previously observed, an A at the first intron nucleotide spliced most efficiently with a 3' splice site UAC. In this context, UAA or UAU were also more efficient 3' splice sites than UAG and competed more efficiently than the wild-type sequence with a 3' splice site UAC. We observed that a U at the first intron nucleotide is used for splicing in combination with 3' splice sites UAG, UAA or UAU. Our data indicate that the 3' splice site is not primarily selected through an interaction with the first intron nucleotide. Selection of the 3' splice site depends critically on its distance from the branchpoint but does not occur by a simple leaky scanning mechanism.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.