ArticleThe Journal of clinical investigation1997
Shear stress induction of the tissue factor gene.
Article in The Journal of clinical investigation, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 236 citations in OpenAlex.
- Mechanobiology of cancer-associated thrombosis: from molecular mechanisms to therapeutic innovation.Journal of nanobiotechnology · 2026Review
- Vascular-type heterogeneity is associated with differential gene expression profiles of endothelial cells under shear stress.Research and practice in thrombosis and haemostasis · 2025Article
- Role of post-translational modifications of Sp1 in cardiovascular diseases.Frontiers in cell and developmental biology · 2024Review
- Hemostasis and Liver Regeneration.Seminars in thrombosis and hemostasis · 2020Review
- Effect of blood flow on platelets, leukocytes, and extracellular vesicles in thrombosis of simulated neonatal extracorporeal circulation.Journal of thrombosis and haemostasis : JTH · 2020Article
- Transport Analysis of Engineered Liver Tissue Fabricated Using a Capsule-Based, Modular Approach.Annals of biomedical engineering · 2019Article
- Intrahepatic fibrin(ogen) deposition drives liver regeneration after partial hepatectomy in mice and humans.Blood · 2019Article
- Endothelial Cell Dysfunction and the Pathobiology of Atherosclerosis.Circulation research · 2016Review
- Blood flow modulation of vascular dynamics.Current opinion in lipidology · 2015Review
- A study of the ultrasound-targeted microbubble destruction based triplex-forming oligodexinucleotide delivery system to inhibit tissue factor expression.Molecular medicine reports · 2015Article
- Regulation of tissue factor gene expression in monocytes and endothelial cells: Thromboxane A2 as a new player.Vascular pharmacology · 2014Article
- Combined effects of physiologically relevant disturbed wall shear stress and glycated albumin on endothelial cell functions associated with inflammation, thrombosis and cytoskeletal dynamics.Journal of diabetes investigation · 2014Article
- Extent of flow recirculation governs expression of atherosclerotic and thrombotic biomarkers in arterial bifurcations.Cardiovascular research · 2014Article
- Transcriptome alterations of mitochondrial and coagulation function in schizophrenia by cortical sequencing analysis.BMC genomics · 2014Article
- Orthostatic hypertension-a new haemodynamic cardiovascular risk factor.Nature reviews. Nephrology · 2013Review
- Impact of stent mis-sizing and mis-positioning on coronary fluid wall shear and intramural stress.Journal of applied physiology (Bethesda, Md. : 1985) · 2013Article
- Hypertensive emergencies are associated with elevated markers of inflammation, coagulation, platelet activation and fibrinolysis.Journal of human hypertension · 2013Article
- Sinomenine protects against lipopolysaccharide-induced acute lung injury in mice via adenosine A(2A) receptor signaling.PloS one · 2013Article
- Coagulation changes during presyncope and recovery.PloS one · 2012Article
- Mis-sizing of stent promotes intimal hyperplasia: impact of endothelial shear and intramural stress.American journal of physiology. Heart and circulatory physiology · 2011Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Using flow channel, we report that the application of a laminar shear stress induced a transient increase of tissue factor (TF) procoagulant activity in human umbilical vein endothelial cells (HUVEC), which was accompanied by a rapid and transient induction of the TF mRNA in the HUVEC. Functional analysis of the 2.2 kb TF 5' promoter indicated that a GC-rich region containing three copies each of the EGR-1 and Sp1 sites was required for induction. Mutation of the Sp1 sites, but not the EGR-1 sites, attenuated the response of TF promoter to shear stress. Thus, Sp1 is a newly defined shear stress responsive element. Electrophoretic mobility shift assays showed there was no increase in binding of nuclear extracts from sheared cells to an Sp1 consensus site. In contrast, immunoblotting of these nuclear extracts with antibody against transcription factor Sp1 demonstrated that shear stress increased the phosphorylation of Sp1. We also showed that shear stress, like the phosphatase inhibitor okadaic acid, increased the transcriptional activity of Sp1. These findings suggest that the shear stress induction of TF gene expression is mediated through an increased Sp1 transcriptional activity with a concomitant hyperphosphorylation of Sp1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.