ArticleJournal of bacteriology1997
Molecular characterization and transcriptional analysis of type 8 capsule genes in Staphylococcus aureus.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
75 citing papers in PubMed, 122 citations in OpenAlex.
- A versatile toolset for Staphylococcus aureus gene inactivation enables testing of anti-phage defense systems in native genetic backgrounds.Microbial cell factories · 2026Article
- Staphylococcal Enterotoxins Promote Virulence in Bacterial Keratitis.Investigative ophthalmology & visual science · 2023Article
- Transcript Profiling of Nitroxoline-Treated Biofilms Shows Rapid Up-regulation of Iron Acquisition Gene Clusters.ACS infectious diseases · 2022Article
- Mesenchymal Stem Cells in the Treatment of New Coronavirus Pandemic: A Novel Promising Therapeutic Approach.Advanced pharmaceutical bulletin · 2022Article
- Inactivation of the Pta-AckA pathway impairs fitness ofJournal of bacteriology · 2021Article
- Pulmonary Pathogens Adapt to Immune Signaling Metabolites in the Airway.Frontiers in immunology · 2020Review
- CidR and CcpA Synergistically Regulate Staphylococcus aureusJournal of bacteriology · 2019Article
- MgrA Negatively Impacts Staphylococcus aureus Invasion by Regulating Capsule and FnbA.Infection and immunity · 2019Article
- Repression of Capsule Production by XdrA and CodY in Staphylococcus aureus.Journal of bacteriology · 2018Article
- MsaB and CodY Interact To Regulate Staphylococcus aureus Capsule in a Nutrient-Dependent Manner.Journal of bacteriology · 2018Article
- Article
- Expression and contribution to virulence of each polysaccharide capsule of Bacillus cereus strain G9241.PloS one · 2018Article
- Article
- Generation of a Stable Plasmid forApplied and environmental microbiology · 2016Article
- MsaB activates capsule production at the transcription level in Staphylococcus aureus.Microbiology (Reading, England) · 2016Article
- SrrAB Modulates Staphylococcus aureus Cell Death through Regulation of cidABC Transcription.Journal of bacteriology · 2016Article
- RbsR Activates Capsule but Represses the rbsUDK Operon in Staphylococcus aureus.Journal of bacteriology · 2015Article
- RNAIII of the Staphylococcus aureus agr system activates global regulator MgrA by stabilizing mRNA.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Examination of the Staphylococcus aureus nitric oxide reductase (saNOR) reveals its contribution to modulating intracellular NO levels and cellular respiration.Molecular microbiology · 2015Article
- Sequence elements upstream of the core promoter are necessary for full transcription of the capsule gene operon in Streptococcus pneumoniae strain D39.Infection and immunity · 2015Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A 20.5-kb contiguous DNA fragment from Staphylococcus aureus Becker affecting type 8 capsule (CP8) biosynthesis was previously cloned. Sequencing analysis indicated that 16 open reading frames (ORFs) encoded within this fragment might be involved in CP8 synthesis. Using various plasmids containing DNA inserts derived from the 20.5-kb region, we showed by complementation of chemical mutants that 8 of the 16 ORFs were required for CP8 synthesis. To determine the involvement of the remaining eight ORFs, nonpolar gene-specific chromosomal mutations located in each of these ORFs were constructed. We found that three additional ORFs were also involved in the CP8 synthesis. Thus, 11 of the 16 ORFs were shown to affect CP8 synthesis. Complementation analyses of these 11 type 8 capsule (cap8) genes affecting CP8 production showed several promoters within the cap8 gene cluster. However, by Northern hybridization using either the entire cap8 gene cluster or the internal fragments of individual ORFs as probes, one 17-kb cap8-specific transcript was detected. Using xylE as the reporter gene, we found that the promoter at the beginning of the cap8 operon was much stronger than any of the internal promoters. These results suggest that the cap8 genes are transcribed mainly as a single large transcript. In addition, Southern hybridization analyses showed that cap8H, cap8I, cap8J, and cap8K, located in the central region of the cap8 gene cluster, were CP8 specific.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.