ArticleJournal of bacteriology1997
Catabolite inactivation of the galactose transporter in the yeast Saccharomyces cerevisiae: ubiquitination, endocytosis, and degradation in the vacuole.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 129 citations in OpenAlex.
- Grape ASR Regulates Glucose Transport, Metabolism and Signaling.International journal of molecular sciences · 2022Article
- Identification of a glucose-insensitive variant of Gal2 from Saccharomyces cerevisiae exhibiting a high pentose transport capacity.Scientific reports · 2021Article
- Endocytosis of nutrient transporters in fungi: The ART of connecting signaling and trafficking.Computational and structural biotechnology journal · 2021Review
- c-di-AMP assists osmoadaptation by regulating theThe Journal of biological chemistry · 2019Article
- Article
- TheBiotechnology for biofuels · 2018Article
- l-Arabinose triggers its own uptake via induction of the arabinose-specific Gal2p transporter in an industrialBiotechnology for biofuels · 2018Article
- The amino-terminal tail of Hxt11 confers membrane stability to the Hxt2 sugar transporter and improves xylose fermentation in the presence of acetic acid.Biotechnology and bioengineering · 2017Article
- Saccharomyces cerevisiae strains for second-generation ethanol production: from academic exploration to industrial implementation.FEMS yeast research · 2017Review
- Multilevel regulation of an α-arrestin by glucose depletion controls hexose transporter endocytosis.The Journal of cell biology · 2017Article
- Regulation of carotenogenesis in the red yeast Xanthophyllomyces dendrorhous: the role of the transcriptional co-repressor complex Cyc8-Tup1 involved in catabolic repression.Microbial cell factories · 2016Article
- Awakening the endogenous Leloir pathway for efficient galactose utilization by Yarrowia lipolytica.Biotechnology for biofuels · 2015Article
- The yeast galactose network as a quantitative model for cellular memory.Molecular bioSystems · 2015Review
- Regulations of sugar transporters: insights from yeast.Current genetics · 2013Review
- Competition between pentoses and glucose during uptake and catabolism in recombinant Saccharomyces cerevisiae.Biotechnology for biofuels · 2012Article
- A molecular switch on an arrestin-like protein relays glucose signaling to transporter endocytosis.The Journal of cell biology · 2012Article
- Article
- Opposing activities of the Snx3-retromer complex and ESCRT proteins mediate regulated cargo sorting at a common endosome.Molecular biology of the cell · 2008Article
- Pathways of glucose regulation of monosaccharide transport in grape cells.Plant physiology · 2006Article
- Immunity to killer toxin K1 is connected with the Golgi-to-vacuole protein degradation pathway.Folia microbiologica · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When Saccharomyces cerevisiae cells growing on galactose are transferred onto glucose medium containing cycloheximide, an inhibitor of protein synthesis, a rapid reduction of Gal2p-mediated galactose uptake is observed. We show that glucose-induced inactivation of Gal2p is due to its degradation. Stabilization of Gal2p in pra1 mutant cells devoid of vacuolar proteinase activity is observed. Subcellular fractionation and indirect immunofluorescence showed that the Gal2 transporter accumulates in the vacuole of the mutant cells, directly demonstrating that its degradation requires vacuolar proteolysis. In contrast, Gal2p degradation is proteasome independent since its half-life is unaffected in pre1-1 pre2-2, cim3-1, and cim5-1 mutants defective in several subunits of the protease complex. In addition, vacuolar delivery of Gal2p was shown to be blocked in conditional end3 and end4 mutants at the nonpermissive temperature, indicating that delivery of Gal2p to the vacuole occurs via the endocytic pathway. Taken together, the results presented here demonstrate that glucose-induced proteolysis of Gal2p is dependent on endocytosis and vacuolar proteolysis and is independent of the functional proteasome. Moreover, we show that Gal2p is ubiquitinated under conditions of glucose-induced inactivation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.