ArticleJournal of bacteriology1997
Suppressor gene analysis reveals an essential role for sphingolipids in transport of glycosylphosphatidylinositol-anchored proteins in Saccharomyces cerevisiae.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 77 citations in OpenAlex.
- Sphingolipid and methionine metabolism in aging.Journal of cell science · 2025Review
- Article
- The yeast sphingolipid signaling landscape.Chemistry and physics of lipids · 2014Review
- Roles for sphingolipids in Saccharomyces cerevisiae.Advances in experimental medicine and biology · 2010Review
- STOP1 regulates multiple genes that protect arabidopsis from proton and aluminum toxicities.Plant physiology · 2009Article
- Yeast sphingolipids: recent developments in understanding biosynthesis, regulation, and function.Biochimica et biophysica acta · 2007Review
- The yeast PH domain proteins Slm1 and Slm2 are targets of sphingolipid signaling during the response to heat stress.Molecular and cellular biology · 2007Article
- Yeast sphingolipids do not need to contain very long chain fatty acids.The Biochemical journal · 2007Article
- GUP1 of Saccharomyces cerevisiae encodes an O-acyltransferase involved in remodeling of the GPI anchor.Molecular biology of the cell · 2006Article
- PGAP2 is essential for correct processing and stable expression of GPI-anchored proteins.Molecular biology of the cell · 2006Article
- Plasma membrane proton ATPase Pma1p requires raft association for surface delivery in yeast.Molecular biology of the cell · 2001Article
- Lag1p and Lac1p are essential for the Acyl-CoA-dependent ceramide synthase reaction in Saccharomyces cerevisae.Molecular biology of the cell · 2001Article
- Coordination between fission yeast glucan formation and growth requires a sphingolipase activity.Genetics · 2001Article
- Ergosterol is required for the Sec18/ATP-dependent priming step of homotypic vacuole fusion.The EMBO journal · 2001Article
- C26-CoA-dependent ceramide synthesis of Saccharomyces cerevisiae is operated by Lag1p and Lac1p.The EMBO journal · 2001Article
- Human antibodies against a purified glucosylceramide from Cryptococcus neoformans inhibit cell budding and fungal growth.Infection and immunity · 2000Article
- A gene encoding a sphingolipid biosynthesis enzyme determines the sensitivity of Saccharomyces cerevisiae to an antifungal plant defensin from dahlia (Dahlia merckii).Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- A longevity assurance gene homolog of tomato mediates resistance to Alternaria alternata f. sp. lycopersici toxins and fumonisin B1.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Lipid rafts function in biosynthetic delivery of proteins to the cell surface in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Two endoplasmic reticulum (ER) membrane proteins that facilitate ER-to-Golgi transport of glycosylphosphatidylinositol-anchored proteins.Molecular biology of the cell · 1999Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Sphingolipids are normally necessary for growth of Saccharomyces cerevisiae cells, but mutant strains that bypass the need for sphingolipids have been identified. Such bypass mutants fail to grow under stressful conditions, including low pH (pH 4.1), when they lack sphingolipids. To begin to understand why sphingolipids seem to be necessary for coping with low-pH stress, we screened a genomic library and selected a suppressor gene, CWP2 (cell wall protein 2), that when present in multiple copies partially compensates for the lack of sphingolipids and enhances survival at low pH. To explain these results, we present evidence that sphingolipids are required for a normal rate of transport of glycosylphosphatidylinositol (GPI)-anchored proteins, including Cwp2 and Gas1/Gpg1, from the endoplasmic reticulum (ER) to the Golgi apparatus. The effect of sphingolipids is specific for transport of GPI-anchored proteins because no effect on the rate of transport of carboxypeptidase Y, a non-GPI-anchored protein, was observed. Since the Gasl protein accumulated in the ER with a GPI anchor in cells lacking sphingolipids, we conclude that sphingolipids are not necessary for anchor attachment. Therefore, sphingolipids must be necessary for a step in formation of COPII vesicles or for their transport to the Golgi apparatus. Our data identify the Cwp2 protein as a vital component in protecting cells from the stress of low pH.
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