ArticleInfection and immunity1997
Identification of the N-acetylneuraminyllactose-specific laminin-binding protein of Helicobacter pylori.
Article in Infection and immunity, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 89 citations in OpenAlex.
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- Modeling and optimization of culture media for recombinantFrontiers in bioengineering and biotechnology · 2024Article
- Bacterial colonization and TH17 immunity are shaped by intestinal sialylation in neonatal mice.Glycobiology · 2022Article
- Extracellular Matrix Interactions with Gram-Positive Pathogens.Microbiology spectrum · 2019Review
- Strategies used byWorld journal of gastroenterology · 2017Review
- Anti-World journal of gastrointestinal pathophysiology · 2016Article
- Antiadhesive properties of arabinogalactan protein from ribes nigrum seeds against bacterial adhesion of Helicobacter pylori.Molecules (Basel, Switzerland) · 2014Article
- Oral immunization with recombinant Lactobacillus acidophilus expressing the adhesin Hp0410 of Helicobacter pylori induces mucosal and systemic immune responses.Clinical and vaccine immunology : CVI · 2014Article
- Antiadhesive properties of Abelmoschus esculentus (Okra) immature fruit extract against Helicobacter pylori adhesion.PloS one · 2014Article
- The rOmp22-HpaA fusion protein confers protective immunity against helicobacter pylori in mice.Current microbiology · 2013Article
- Article
- Helicobacter pylori AlpA and AlpB bind host laminin and influence gastric inflammation in gerbils.Infection and immunity · 2011Article
- Avian pathogenic Escherichia coli bind fibronectin and laminin.Veterinary research communications · 2009Article
- Laminin isoforms in development and disease.Journal of molecular medicine (Berlin, Germany) · 2007Review
- A newly identified leptospiral adhesin mediates attachment to laminin.Infection and immunity · 2006Article
- Pathogenesis of Helicobacter pylori infection.Clinical microbiology reviews · 2006Review
- B-cell and T-cell immune responses to experimental Helicobacter pylori infection in humans.Infection and immunity · 2005Article
- Identification of a Treponema pallidum laminin-binding protein.Infection and immunity · 2003Article
- Helicobacter pylori interactions with host serum and extracellular matrix proteins: potential role in the infectious process.Microbiology and molecular biology reviews : MMBR · 2002Review
- Helicobacter pylori-specific immune responses of children: implications for future vaccination strategy.Clinical and diagnostic laboratory immunology · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The interaction of the gastroduodenal pathogen Helicobacter pylori with the glycoprotein laminin was investigated. Binding of 125I-radiolabelled laminin in a liquid-phase assay by both hemagglutinating and poorly hemagglutinating strains was rapid, saturable, specific, partially reversible, of high affinity, and insensitive to pH. Inhibition of laminin binding by fetuin, but not asialofetuin, and reduced bacterial binding to periodate- or sialidase-treated laminin indicated that glycosylation, particularly sialylation, was important for laminin binding by H. pylori. Inhibition experiments with monosaccharides, disaccharides, and trisaccharides showed that the strains bound to a region spanning a trisaccharide. In particular, inhibition and displacement studies showed that binding to the trisaccharide N-acetylneuraminyl-alpha(2-3)-lactose [NeuAc(2-3)Lac] was preferential to that to the NeuAc(2-6)Lac isomer. Complete inhibition of laminin binding by both hemagglutinating and poorly hemagglutinating strains was achieved only when isolated lipopolysaccharide (LPS) was used as an inhibitor in combination with heat or protease treatment of H. pylori cells, thereby confirming the involvement of both LPS and a protein adhesin in laminin binding. Further inhibition experiments indicated that the protein receptor, rather than LPS, on H. pylori bound NeuAc(2-3)Lac. By using a Western blotting procedure, a 25-kDa outer membrane protein was identified as mediating laminin binding by both hemagglutinating and poorly hemagglutinating H. pylori strains. The specificity of binding was confirmed by complete inhibition of laminin binding by the 25-kDa protein with NeuAc(2-3)Lac. The data collectively suggest that a 25-kDa outer membrane protein acts in a lectin-like manner with LPS to mediate attachment of H. pylori to laminin.
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