ArticlePlant molecular biology1997
Mutations of cytochrome b6 in Chlamydomonas reinhardtii disclose the functional significance for a proline to leucine conversion by petB editing in maize and tobacco.
Article in Plant molecular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 78 citations in OpenAlex.
- P-Type Pentatricopeptide Repeat Proteins YS1 and YS2 Function in Splicing ofInternational journal of molecular sciences · 2025Article
- Emergence of Novel RNA-Editing Sites by Changes in the Binding Affinity of a Conserved PPR Protein.Molecular biology and evolution · 2022Article
- Juggling Lightning: How Chlorella ohadii handles extreme energy inputs without damage.Photosynthesis research · 2021Article
- The complete mitochondrial genome of Cycas debaoensis revealed unexpected static evolution in gymnosperm species.PloS one · 2021Article
- Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in Soybean.International journal of molecular sciences · 2020Article
- Chloroplast Translation: Structural and Functional Organization, Operational Control, and Regulation.The Plant cell · 2018Review
- Identification and Analysis of RNA Editing Sites in the Chloroplast Transcripts of Aegilops tauschii L.Genes · 2016Article
- Dynamics of Chloroplast Translation during Chloroplast Differentiation in Maize.PLoS genetics · 2016Article
- Spontaneous dominant mutations in chlamydomonas highlight ongoing evolution by gene diversification.The Plant cell · 2015Article
- Massive gene transfer and extensive RNA editing of a symbiotic dinoflagellate plastid genome.Genome biology and evolution · 2014Article
- Collapsing aged culture of the cyanobacterium Synechococcus elongatus produces compound(s) toxic to photosynthetic organisms.PloS one · 2014Article
- Differential regulation of Arabidopsis plastid gene expression and RNA editing in non-photosynthetic tissues.Plant molecular biology · 2013Article
- An RNA recognition motif-containing protein is required for plastid RNA editing in Arabidopsis and maize.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- How to build functional thylakoid membranes: from plastid transcription to protein complex assembly.Planta · 2013Review
- Review
- RESOPS: a database for analyzing the correspondence of RNA editing sites to protein three-dimensional structures.Plant & cell physiology · 2009Article
- Is the redox state of the ci heme of the cytochrome b6f complex dependent on the occupation and structure of the Qi site and vice versa?The Journal of biological chemistry · 2009Article
- Post-transcriptional control of chloroplast gene expression.Gene regulation and systems biology · 2009Article
- Correlation between amino acid residues converted by RNA editing and functional residues in protein three-dimensional structures in plant organelles.BMC plant biology · 2008Article
- Cross-competition in editing of chloroplast RNA transcripts in vitro implicates sharing of trans-factors between different C targets.The Journal of biological chemistry · 2008Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have introduced a proline codon in place of a leucine codon at position 204 of the petB gene of Chlamydomonas reinhardtii. This gene modification mimics the presence of proline codons at the same position in the petB genes of maize and tobacco, which are subsequently edited to leucine codons at the RNA level. Following transformation, we observed no editing at this position in C. reinhardtii, independent of the type of proline codon we have used: the CCA codon, edited in maize, or a CCT codon. Strains carrying the introduced mutation were non phototrophic and displayed a block in photosynthetic electron transfer, consistent with a lack of cytochrome b6f activity. Thus the presence of a proline residue at position 204 in cytochrome b6 is detrimental to photosynthesis. We show that the mutant phenotype arose from a defective assembly of cytochrome b6f complexes and not from altered electron transfer properties in the assembled protein complex. Biochemical comparison of the proline-containing transformants with a cytochrome b6 mutant deficient in heme-attachment indicates that their primary defect is at the level of assembly of apocytochrome b6 with the bh heme, thereby preventing assembly of the whole cytochrome b6f complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.