ArticleProceedings of the National Academy of Sciences of the United States of America1997
An unusual histone H3 specific for early macronuclear development in Euplotes crassus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 32 citations in OpenAlex.
- Programmed chromosome fragmentation in ciliated protozoa: multiple means to chromosome ends.Microbiology and molecular biology reviews : MMBR · 2023Review
- Multilevel interrogation of H3.3 reveals a primordial role in transcription regulation.Epigenetics & chromatin · 2023Article
- Article
- Differential expression of histone H3 genes and selective association of the variant H3.7 with a specific sequence class in Stylonychia macronuclear development.Epigenetics & chromatin · 2014Article
- Developmentally regulated chromosome fragmentation linked to imprecise elimination of repeated sequences in paramecia.Eukaryotic cell · 2003Article
- Role of histone deacetylation in developmentally programmed DNA rearrangements in Tetrahymena thermophila.Eukaryotic cell · 2002Article
- De novo telomere addition to spacer sequences prior to their developmental degradation in Euplotes crassus.Nucleic acids research · 2002Article
- Differentiation of chromatin during DNA elimination in Euplotes crassus.Molecular biology of the cell · 1999Article
- Conserved DNA sequences adjacent to chromosome fragmentation and telomere addition sites in Euplotes crassus.Nucleic acids research · 1998Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Characterization of the histone H3 genes of the ciliated protozoan Euplotes crassus indicates that one gene functions only during the sexual phase of the life cycle. Maximum expression of this gene, as judged by transcript accumulation, correlates with DNA replications leading to polytenization of the micronuclear chromosomes before massive DNA elimination, which produces a transcriptionally active macronucleus. Transcripts of the other gene accumulate primarily during vegetative growth and in the sexual phase of the life cycle during replication phases not related to polytenization. Although both histone H3 genes encode proteins that are fairly divergent in sequence at the amino terminus, the meiotic/polytene-specific histone H3 contains two insertions in the amino terminus that increase the size of the protein by 15 amino acids. Analysis of micrococcal nuclease digests of chromatin using hybridization probes specific for micronuclear vs. macronuclear sequences indicates that a change in nucleosomal spacing correlates with the maximal expression of the meiotic/polytene-specific histone H3 gene. Thus, we surmise that this unusual histone H3 may play a key role in targeting DNA sequences for either transcriptional activation and retention in the macronucleus or heterochromatization and elimination.
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