ArticleProceedings of the National Academy of Sciences of the United States of America1997
p21-activated kinase has substrate specificity similar to Acanthamoeba myosin I heavy chain kinase and activates Acanthamoeba myosin I.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 81 citations in OpenAlex.
- Endocytic myosin-1 is a force-insensitive, power-generating motor.The Journal of cell biology · 2023Article
- Endocytic myosin-1 is a force-insensitive, power-generating motor.bioRxiv : the preprint server for biology · 2023Article
- Cdc42/Rac Interactive Binding Containing Effector Proteins in Unicellular Protozoans With Reference to Human Host: Locks of the Rho Signaling.Frontiers in genetics · 2022Review
- Myosin XVI in the Nervous System.Cells · 2020Review
- Unconventional Myosins: How Regulation Meets Function.International journal of molecular sciences · 2019Review
- Various Themes of Myosin Regulation.Journal of molecular biology · 2016Review
- Components of the CtBP1/BARS-dependent fission machinery.Histochemistry and cell biology · 2013Review
- Chk1 phosphorylation of Metnase enhances DNA repair but inhibits replication fork restart.Oncogene · 2012Article
- Potential roles of myosin VI in cell motility.Biochemical Society transactions · 2009Review
- How are the cellular functions of myosin VI regulated within the cell?Biochemical and biophysical research communications · 2008Review
- p21-activated kinase signaling in breast cancer.Breast cancer research : BCR · 2005Review
- Myosin VI: cellular functions and motor properties.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2004Review
- Akt phosphorylation of serine 21 on Pak1 modulates Nck binding and cell migration.Molecular and cellular biology · 2003Article
- Regulation of macropinocytosis by p21-activated kinase-1.Molecular biology of the cell · 2000Article
- KFC, a Ste20-like kinase with mitogenic potential and capability to activate the SAPK/JNK pathway.Oncogene · 2000Article
- Direct involvement of yeast type I myosins in Cdc42-dependent actin polymerization.The Journal of cell biology · 2000Article
- PAKa, a putative PAK family member, is required for cytokinesis and the regulation of the cytoskeleton in Dictyostelium discoideum cells during chemotaxis.The Journal of cell biology · 1999Article
- p21-activated kinase 1 (Pak1) regulates cell motility in mammalian fibroblasts.The Journal of cell biology · 1999Article
- Article
- Myosin I heavy chain kinase: cloning of the full-length gene and acidic lipid-dependent activation by Rac and Cdc42.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Acanthamoeba class I myosins are unconventional, single-headed myosins that express actin-activated Mg2+-ATPase and in vitro motility activities only when a single serine or threonine in the heavy chain is phosphorylated by myosin I heavy chain kinase (MIHCK). Some other, but not most, class I myosins have the same consensus phosphorylation site sequence, and the two known class VI myosins have a phosphorylatable residue in the homologous position, where most myosins have an aspartate or glutamate residue. Recently, we found that the catalytic domain of Acanthamoeba MIHCK has extensive sequence similarity to the p21-activated kinase (PAK)/STE20 family of kinases from mammals and yeast, which are activated by small GTP-binding proteins. The physiological substrates of the PAK/STE20 kinases are not well characterized. In this paper we show that PAK1 has similar substrate specificity as MIHCK when assayed against synthetic substrates and that PAK1 phosphorylates the heavy chain (1 mol of P(i) per mol) and activates Acanthamoeba myosin I as MIHCK does. These results, together with the known involvement of Acanthamoeba myosin I, yeast myosin I, STE20, PAK, and small GTP-binding proteins in membrane- and cytoskeleton-associated morphogenetic transformations and activities, suggest that myosins may be physiological substrates for the PAK/STE20 family and thus mediators of these events.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.