ArticleJournal of immunology (Baltimore, Md. : 1950)1997
VHDJH gene sequences and antigen reactivity of monoclonal antibodies produced by human B-1 cells: evidence for somatic selection.
Article in Journal of immunology (Baltimore, Md. : 1950), 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 59 citations in OpenAlex.
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- Clonal redemption of B cells in cancer.Frontiers in immunology · 2023Article
- Chronic Lymphocytic Leukemia B-Cell Normal Cellular Counterpart: Clues From a Functional Perspective.Frontiers in immunology · 2018Review
- Polyreactive natural antibodies in transplantation.Current opinion in organ transplantation · 2017Review
- Natural and adaptive IgM antibodies in the recognition of tumor-associated antigens of breast cancer (Review).Oncology reports · 2015Review
- Human B-cell ontogeny in humanized NOD/SCID γc(null) mice generates a diverse yet auto/poly- and HIV-1-reactive antibody repertoire.Genes and immunity · 2012Article
- Chronic inflammatory disease, lymphoid tissue neogenesis and extranodal marginal zone B-cell lymphomas.Haematologica · 2009Review
- Nature and functions of autoantibodies.Nature clinical practice. Rheumatology · 2008Review
- Natural human antibodies to pneumococcus have distinctive molecular characteristics and protect against pneumococcal disease.Clinical and experimental immunology · 2008Article
- Autoantigen, innate immunity, and T cells cooperate to break B cell tolerance during bacterial infection.The Journal of clinical investigation · 2005Article
- Chronic lymphocytic leukemia B cells can undergo somatic hypermutation and intraclonal immunoglobulin V(H)DJ(H) gene diversification.The Journal of experimental medicine · 2002Article
- Regulation and chance in the ontogeny of B and T cell antigen receptor repertoires.Immunologic research · 2002Review
- Clonal evolution of B cells in transformation from low- to high-grade lymphoma.European journal of immunology · 1999Article
- Induction of Ig somatic hypermutation and class switching in a human monoclonal IgM+ IgD+ B cell line in vitro: definition of the requirements and modalities of hypermutation.Journal of immunology (Baltimore, Md. : 1950) · 1999Article
- Article
- Clonally-related immunoglobulin VH domains and nonrandom use of DH gene segments in rheumatoid arthritis synovium.Molecular medicine (Cambridge, Mass.) · 1998Article
- Lack of intraclonal diversification in Ig heavy and light chain V region genes expressed by CD5+IgM+ chronic lymphocytic leukemia B cells: a multiple time point analysis.Journal of immunology (Baltimore, Md. : 1950) · 1998Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
To understand whether the distinct VHDJH gene utilization by natural polyreactive Abs reflects the developmentally restricted Ig VHDJH rearrangements putatively expressed by B-1 cells, we generated 11 (8 IgM, 1 IgG3, 2 IgA1), 7 (6 IgM, 1 IgG1), and 7 (2 IgM, 3 IgG1, 2 IgG3) mAb-producing lines using B-1a (surface CD5+, CD45RAlow), B-1b (surface CD5-, CD45RAlow, CD5 mRNA+), and B-2 (surface CD5-, CD45RAhigh, CD5 mRNA-) cells, respectively, sorted from adult human peripheral blood. Most B-1a and B-1b, but no B-2, cell-derived mAbs were polyreactive; i.e., they bound different self and foreign Ags with different affinities. B-1a and B-2 mAbs preferentially utilized VH4 (p = 0.003) and VH3 (p = 0.010) genes, respectively. All three mAb populations utilized DXP, DLR, DN DH genes, and JH6, but no mAb utilized DHQ52. There were fewer unencoded nucleotide (N) additions in the VHDJH junctions of B-1b (3.00 +/- 2.52, mean +/- SD) than of B-1a (12.45 +/- 3.93, p = 1.23 x 10(-5)) or B-2 (8.29 +/- 4.75, p = 0.020) mAbs. Partly due to the fewer N additions and a paucity of D-D fusions, the B-1b mAb CDR3s were significantly shorter than the B-1a mAb CDR3s (p = 0.013), which contained a nonrandom Tyr distribution (p = 0.003). Finally, all but two B-1 cell-derived mAbs were mutated, in a fashion similar to that of the Ag-selected B-2 mAbs. Thus, in the human adult, B-1 cells that make natural polyreactive Abs may not be representative of the predominantly B-1 developmental waves of colonization of the fetal and neonatal B cell repertoires, and are somatically selected.
Indexed as
Identifiers
9037000PMC4631314W1519282847What OpenQuestion holds
Registered trials
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