ArticleThe EMBO journal1997
The mammalian profilin isoforms display complementary affinities for PIP2 and proline-rich sequences.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 141 citations in OpenAlex.
- Molecular insights into profilin 1-dependent regulation of cellular phosphatidylinositol (4,5)-bisphosphate.Journal of cell science · 2026Article
- Reconstituted nascent adhesion condensates drive actin polymerization on supported lipid bilayers.Science advances · 2026Article
- Molecular insights into Profilin1-dependent regulation of cellular phosphatidylinositol-(4,5)-bisphosphate.bioRxiv : the preprint server for biology · 2025Article
- Actin-binding protein profilin1 is an important determinant of cellular phosphoinositide control.The Journal of biological chemistry · 2024Article
- Phosphorylation of the actin-binding protein profilin2a at S137 modulates bidirectional structural plasticity at dendritic spines.Frontiers in cell and developmental biology · 2023Article
- The role of profilin-1 in cardiovascular diseases.Journal of cell science · 2021Review
- Profilin Isoforms in Health and Disease - All the Same but Different.Frontiers in cell and developmental biology · 2021Review
- PFN2 and NAA80 cooperate to efficiently acetylate the N-terminus of actin.The Journal of biological chemistry · 2020Article
- Profilin choreographs actin and microtubules in cells and cancer.International review of cell and molecular biology · 2020Article
- Emerging Functions of Actins and Actin Binding Proteins in Trypanosomatids.Frontiers in cell and developmental biology · 2020Review
- ATP binds and inhibits the neurodegeneration-associated fibrillization of the FUS RRM domain.Communications biology · 2019Article
- Cofilin and profilin: partners in cancer aggressiveness.Biophysical reviews · 2018Review
- Structural basis for mutation-induced destabilization of profilin 1 in ALS.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Fission yeast profilin is tailored to facilitate actin assembly by the cytokinesis formin Cdc12.Molecular biology of the cell · 2015Article
- The multifaceted role of profilin-1 in adipose tissue inflammation and glucose homeostasis.Adipocyte · 2014Article
- The role of actin filament dynamics in the myogenic response of cerebral resistance arteries.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2013Review
- Differential remodeling of actin cytoskeleton architecture by profilin isoforms leads to distinct effects on cell migration and invasion.Cancer cell · 2012Article
- Characterization of profilin polymorphism in pollen with a focus on multifunctionality.PloS one · 2012Article
- Fine-tuning of neuronal architecture requires two profilin isoforms.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- A central role for the WH2 domain of Srv2/CAP in recharging actin monomers to drive actin turnover in vitro and in vivo.Cytoskeleton (Hoboken, N.J.) · 2010Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a study on the binding properties of the bovine profilin isoforms to both phosphatidylinositol 4,5-bisphosphate (PIP2) and proline-rich peptides derived from vasodilator-stimulated phosphoprotein (VASP) and cyclase-associated protein (CAP). Using microfiltration, we show that compared with profilin II, profilin I has a higher affinity for PIP2. On the other hand, fluorescence spectroscopy reveals that proline-rich peptides bind better to profilin II. At micromolar concentrations, profilin II dimerizes upon binding to proline-rich peptides. Circular dichroism measurements of profilin II reveal a significant conformational change in this protein upon binding of the peptide. We show further that PIP2 effectively competes for binding of profilin I to poly-L-proline, since this isoform, but not profilin II, can be eluted from a poly-L-proline column with PIP2. Using affinity chromatography on either profilin isoform, we identified profilin II as the preferred ligand for VASP in bovine brain extracts. The complementary affinities of the profilin isoforms for PIP2 and the proline-rich peptides offer the cell an opportunity to direct actin assembly at different subcellular localizations through the same or different signal transduction pathways.
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