ArticleThe Biochemical journal1997
Purification and characterization of paraoxon hydrolase from rat liver.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 60 citations in OpenAlex.
- Highly Dynamic Gene Family Evolution Suggests Changing Roles for PON Genes Within Metazoa.Genome biology and evolution · 2023Article
- Serum paraoxonase 1 activity is paradoxically maintained in nonalcoholic fatty liver disease despite low HDL cholesterol.Journal of lipid research · 2019Article
- Protective effect of rutin on the antioxidant genes expression in hypercholestrolemic male Westar rat.BMC complementary and alternative medicine · 2013Article
- Effects of orange juice and hesperetin on serum paraoxonase activity and lipid profile in hyperuricemic rats.BioImpacts : BI · 2012Article
- Measurement of serum paraoxonase-1 activity in the evaluation of liver function.World journal of gastroenterology · 2009Article
- Paraoxonase-1 is related to inflammation, fibrosis and PPAR delta in experimental liver disease.BMC gastroenterology · 2009Article
- Time-dependent modulation of rat serum paraoxonase 1 activity by fasting.Pflugers Archiv : European journal of physiology · 2007Article
- Identification of paraoxonase 3 in rat liver microsomes: purification and biochemical properties.The Biochemical journal · 2003Article
- Hydrolysis of platelet-activating factor by human serum paraoxonase.The Biochemical journal · 2001Article
- Isolation and complete covalent structure of liver microsomal paraoxonase.The Biochemical journal · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Paraoxonase (paraoxon hydrolase), an enzyme that hydrolyses paraoxon (O,O-diethyl O-p-nitrophenyl phosphate), is located in mammals primarily in the serum and liver. Although considerable information is available regarding serum paraoxonase, little is known about the hepatic form of this enzyme. The present work represents the first study on the purification of rat liver paraoxonase. This enzyme has been purified 415-fold to apparent homogeneity with a final specific activity of 1370 units/mg using a protocol consisting of five steps: solubilization of the microsomal fraction, hydroxyapatite adsorption, chromatography on DEAE-Sepharose CL-6B, non-specific affinity chromatography on Cibacron Blue 3GA and anion exchange on Mono Q HR 5/5. The presence of Ca2+ and Triton X-100 in the buffers throughout the purification procedure was essential for maintaining enzyme activity. SDS/PAGE of the final preparation indicated a single protein-staining band with an apparent Mr of 45 000. N-terminal and internal amino acid sequences were determined and compared with those of paraoxonases from human and rabbit serum and mouse liver, showing a high similarity. The pH profile showed optimum activity at pH 8.5. The pH stability and heat inactivation of the enzyme were also studied. The Km for liver paraoxonase was 1.69 mM.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.