ArticleMolecular and cellular biology1997
A globin enhancer acts by increasing the proportion of erythrocytes expressing a linked transgene.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 75 citations in OpenAlex.
- Power-law behavior of transcriptional bursting regulated by enhancer-promoter communication.Genome research · 2024Article
- Chromatin remodeling effects on enhancer activity.Cellular and molecular life sciences : CMLS · 2016Review
- The use of mouse models to study epigenetics.Cold Spring Harbor perspectives in biology · 2013Review
- Remission of diabetes by insulin gene therapy using a hepatocyte-specific and glucose-responsive synthetic promoter.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- The GATA1-HS2 enhancer allows persistent and position-independent expression of a β-globin transgene.PloS one · 2011Article
- Both the constitutive cauliflower mosaic virus 35S and tissue-specific AGAMOUS enhancers activate transcription autonomously in Arabidopsis thaliana.Plant molecular biology · 2010Article
- Modeling reveals bistability and low-pass filtering in the network module determining blood stem cell fate.PLoS computational biology · 2010Article
- Increased p53 activity does not accelerate telomere-driven ageing.EMBO reports · 2006Article
- Transcriptional regulation of the SCL locus: identification of an enhancer that targets the primitive erythroid lineage in vivo.Molecular and cellular biology · 2005Article
- Stochasticity or the fatal 'imperfection' of cloning.Journal of biosciences · 2005Review
- Mono-allelic expression of variegating transgene locus in the mouse.Transgenic research · 2003Article
- Chromatin insulation by a transcriptional activator.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Article
- Quantitative analysis of globin gene induction in single human erythroleukemic cells.Nucleic acids research · 2000Article
- Complex patterns of inheritance of an imprinted murine transgene suggest incomplete germline erasure.Nucleic acids research · 2000Article
- Variegation associated with lacZ in transgenic animals: a warning note.Transgenic research · 2000Article
- Gene therapy for the hemoglobin disorders: past, present, and future.Proceedings of the National Academy of Sciences of the United States of America · 2000Review
- Enhancement of beta-globin locus control region-mediated transactivation by mitogen-activated protein kinases through stochastic and graded mechanisms.Molecular and cellular biology · 1999Article
- Enhancer-dependent transcriptional oscillations in mouse erythroleukemia cells.Molecular and cellular biology · 1999Article
- The chicken beta-globin 5'HS4 boundary element blocks enhancer-mediated suppression of silencing.Molecular and cellular biology · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Enhancer elements have been shown to affect the probability of a gene establishing an active transcriptional state and suppress the silencing of reporter genes in cell lines, but their effect in transgenic mice has been obscured by the use of assays that do not assess expression on a cell-by-cell basis. We have examined the effect of a globin enhancer on the variegation of lacZ expression in erythrocytes of transgenic mice. Mice carrying lacZ driven by the alpha-globin promoter exhibit beta-galactosidase (beta-Gal) expression in only a very small proportion of embryonic erythrocytes. When the transgenic construct also contains the (alphaHS-40 enhancer, which controls expression of the alpha-globin gene, expression is seen in a high proportion of embryonic erythrocytes, although there are variations between transgenic lines which can be attributed to different sites of integration. Analysis of beta-Gal expression levels suggests that expressing cells in lines carrying only the alpha-globin promoter express as much beta-Gal as those in which the transgene also contains alphaHS-40. A marked decline in transgene expression occurs as mice age, which is mainly due to a decrease in the proportion of cells expressing the transgene. Thus, a globin enhancer can act to suppress variegation of a linked transgene; this result is consistent with a model in which enhancers act to establish and maintain an active domain without directly affecting the transcriptional rate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.