ArticleMolecular and cellular biology1997
Cloning and characterization of Ras-GRF2, a novel guanine nucleotide exchange factor for Ras.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 129 citations in OpenAlex.
- High expression of Ras-specific guanine nucleotide-releasing factor 2 (RasGRF2) in lung adenocarcinoma is associated with tumor invasion and poor prognosis.Pathology international · 2021Article
- MG132 exerts anti-viral activity against HSV-1 by overcoming virus-mediated suppression of the ERK signaling pathway.Scientific reports · 2020Article
- Synaptic GluN2A-Containing NMDA Receptors: From Physiology to Pathological Synaptic Plasticity.International journal of molecular sciences · 2020Review
- RasGRF2 promotes migration and invasion of colorectal cancer cells by modulating expression of MMP9 through Src/Akt/NF-κB pathway.Cancer biology & therapy · 2019Article
- A Histidine pH sensor regulates activation of the Ras-specific guanine nucleotide exchange factor RasGRP1.eLife · 2017Article
- Transcriptional profiling reveals functional links between RasGrf1 and Pttg1 in pancreatic beta cells.BMC genomics · 2014Article
- R-Ras contributes to LTP and contextual discrimination.Neuroscience · 2014Article
- Function of RasGRP3 in the formation and progression of human breast cancer.Molecular cancer · 2014Article
- RhoGEFs in cell motility: novel links between Rgnef and focal adhesion kinase.Current molecular medicine · 2014Review
- RASGRF2 regulates alcohol-induced reinforcement by influencing mesolimbic dopamine neuron activity and dopamine release.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Ca-dependent Ras/Erk signaling mediates negative selection of autoreactive B cells.Small GTPases · 2011Article
- RasGRF suppresses Cdc42-mediated tumour cell movement, cytoskeletal dynamics and transformation.Nature cell biology · 2011Article
- Regulation of Neuronal Function by Ras-GRF Exchange Factors.Genes & cancer · 2011Article
- Amphetamine alters Ras-guanine nucleotide-releasing factor expression in the rat striatum in vivo.European journal of pharmacology · 2009Article
- Structural and spatial determinants regulating TC21 activation by RasGRF family nucleotide exchange factors.Molecular biology of the cell · 2009Article
- GEF1 is a ciliary Sec7 GEF of Tetrahymena thermophila.Cell motility and the cytoskeleton · 2009Article
- Transgenerational rescue of a genetic defect in long-term potentiation and memory formation by juvenile enrichment.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- NMDA receptor stimulation induces reversible fission of the neuronal endoplasmic reticulum.PloS one · 2009Article
- RasGRF2, a guanosine nucleotide exchange factor for Ras GTPases, participates in T-cell signaling responses.Molecular and cellular biology · 2007Article
- Distinct roles for Ras-guanine nucleotide-releasing factor 1 (Ras-GRF1) and Ras-GRF2 in the induction of long-term potentiation and long-term depression.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conversion of Ras proteins into an activated GTP-bound state able to bind effector proteins is catalyzed by specific guanine nucleotide exchange factors in response to a large number of extracellular stimuli. Here we report the isolation of mouse cDNAs encoding Ras-GRF2, a multidomain 135-kDa protein containing a COOH-terminal Cdc25-related domain that stimulates release of GDP from Ras but not other GTPases in vitro. Ras-GRF2 bound specifically to immobilized Ras lacking bound nucleotides, suggesting stabilization of the nucleotide-free form of Ras as a mechanism of catalyzing nucleotide exchange. The NH2-terminal region of Ras-GRF2 is predicted to contain features common to various signaling proteins including two pleckstrin homology domains and a Dbl homology region. Ras-GRF2 also contains an IQ motif which was required for its apparent constitutive association with calmodulin in epithelial cells ectopically expressing Ras-GRF2. Transient expression of Ras-GRF2 in kidney epithelial cells stimulated GTP binding by Ras and potentiated calcium ionophore-induced activation of mitogen-activated protein kinase (ERK1) dependent upon the IQ motif. Calcium influx caused Ras-GRF2 subcellular localization to change from cytosolic to peripheral, suggesting a possible mechanism for controlling Ras-GRF2 interactions with Ras at the plasma membrane. Epithelial cells overexpressing Ras-GRF2 are morphologically transformed and grow in a disorganized manner with minimal intercellular contacts. Northern analysis indicated a 9-kb GRF2 transcript in brain and lung, where p135 Ras-GRF2 is known to be expressed, and RNAs of 12 kb and 2.2 kb were detected in several tissues. Thus, Ras-GRF2 proteins with different domain structures may be widely expressed and couple diverse extracellular signals to Ras activation.
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