ArticleVirus research1996
Cooperation between transmissible gastroenteritis coronavirus (TGEV) structural proteins in the in vitro induction of virus-specific antibodies.
Article in Virus research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Overview of the current promising approaches for the development of an effective severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine.International immunopharmacology · 2020Review
- Characterization of an Immunodominant Epitope in the Endodomain of the Coronavirus Membrane Protein.Viruses · 2016Article
- A phase trial of the oral Lactobacillus casei vaccine polarizes Th2 cell immunity against transmissible gastroenteritis coronavirus infection.Applied microbiology and biotechnology · 2016Article
- Article
- A recombinant nucleocapsid protein-based indirect enzyme-linked immunosorbent assay to detect antibodies against porcine deltacoronavirus.The Journal of veterinary medical science · 2016Article
- Development of reverse transcription loop-mediated isothermal amplification for rapid detection of porcine epidemic diarrhea virus.Virus genes · 2011Article
- Reverse transcription loop-mediated isothermal amplification for rapid detection of transmissible gastroenteritis virus.Current microbiology · 2011Article
- Cholesterol is important for a post-adsorption step in the entry process of transmissible gastroenteritis virus.Antiviral research · 2010Article
- Enhancing immune responses against SARS-CoV nucleocapsid DNA vaccine by co-inoculating interleukin-2 expressing vector in mice.Biotechnology letters · 2009Article
- Article
- SARS vaccines: where are we?Expert review of vaccines · 2009Review
- Article
- Vaccines to prevent severe acute respiratory syndrome coronavirus-induced disease.Virus research · 2008Article
- Vaccination of mice with recombinant baculovirus expressing spike or nucleocapsid protein of SARS-like coronavirus generates humoral and cellular immune responses.Molecular immunology · 2008Article
- Article
- Article
- Immune responses against SARS-coronavirus nucleocapsid protein induced by DNA vaccine.Virology · 2005Article
- Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice.Vaccine · 2005Article
- A subcutaneously injected UV-inactivated SARS coronavirus vaccine elicits systemic humoral immunity in mice.International immunology · 2004Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Following infection of haplotype defined NIH-miniswine with virulent transmissible gastroenteritis coronavirus (TGEV), isolated mesenteric lymph node CD4+ T-cells mounted a specific proliferative response against infectious or inactivated purified virus in secondary in vitro stimulation. A specific, dose-dependent response to the three major recombinant viral proteins: spike (S), membrane (M), and nucleoprotein (N), purified by affinity chromatography, was characterized. Induction of in vitro antibody synthesis was analyzed. The purified recombinant viral proteins induced the in vitro synthesis of neutralizing TGEV-specific antibodies when porcine TGEV-immune cells were stimulated with each of the combinations made with two of the major structural proteins: S + N, S + M, and to a minor extent with M + N, but not by the individual proteins. S-protein was dissociated from purified virus using NP-40 detergent and then micellar S-protein oligomers (S-rosettes) were formed by removing the detergent. These occurred preferentially by the association of more than 10 S-protein trimmers. These S-rosettes in collaboration with either N or M-proteins elicited TGEV-specific antibodies with titers up to 84 and 60%, respectively, of those induced by the whole virus. N-protein could be partially substituted by a 15-mer peptide that represents a T helper epitope previously identified in N-protein (Antón et al. (1995)). These results indicate that the induction of high levels of TGEV-specific antibodies requires stimulation by at least two viral proteins, and that optimum responses are induced by a combination of S-rosettes and the nucleoprotein.
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