ArticleThe EMBO journal1997
Pop3p is essential for the activity of the RNase MRP and RNase P ribonucleoproteins in vivo.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 82 citations in OpenAlex.
- Menthone lowers H3K27ac levels to inhibitFrontiers in microbiology · 2025Article
- Lifespan Extension by Retrotransposons under Conditions of Mild Stress Requires Genes Involved in tRNA Modifications and Nucleotide Metabolism.International journal of molecular sciences · 2024Article
- Human RNase P: overview of a ribonuclease of interrelated molecular networks and gene-targeting systems.RNA (New York, N.Y.) · 2023Review
- Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation inRNA (New York, N.Y.) · 2019Article
- Post-Translational Dosage Compensation Buffers Genetic Perturbations to Stoichiometry of Protein Complexes.PLoS genetics · 2017Article
- The Diversity of Ribonuclease P: Protein and RNA Catalysts with Analogous Biological Functions.Biomolecules · 2016Review
- Footprinting analysis of interactions between the largest eukaryotic RNase P/MRP protein Pop1 and RNase P/MRP RNA components.RNA (New York, N.Y.) · 2015Article
- GAMETOPHYTE DEFECTIVE 1, a putative subunit of RNases P/MRP, is essential for female gametogenesis and male competence in Arabidopsis.PloS one · 2012Article
- Assembly of Saccharomyces cerevisiae 60S ribosomal subunits: role of factors required for 27S pre-rRNA processing.The EMBO journal · 2011Article
- Of proteins and RNA: the RNase P/MRP family.RNA (New York, N.Y.) · 2010Review
- Crystallization and preliminary X-ray diffraction analysis of the P3 RNA domain of yeast ribonuclease MRP in a complex with RNase P/MRP protein components Pop6 and Pop7.Acta crystallographica. Section F, Structural biology and crystallization communications · 2010Article
- 60S ribosomal subunit assembly dynamics defined by semi-quantitative mass spectrometry of purified complexes.Nucleic acids research · 2008Article
- Interactions between subunits of Saccharomyces cerevisiae RNase MRP support a conserved eukaryotic RNase P/MRP architecture.Nucleic acids research · 2007Article
- Inventory and analysis of the protein subunits of the ribonucleases P and MRP provides further evidence of homology between the yeast and human enzymes.Nucleic acids research · 2006Article
- 3D models of yeast RNase P/MRP proteins Rpp1p and Pop3p.RNA (New York, N.Y.) · 2005Article
- Diazaborine treatment of yeast cells inhibits maturation of the 60S ribosomal subunit.Molecular and cellular biology · 2004Article
- RNase MRP cleaves the CLB2 mRNA to promote cell cycle progression: novel method of mRNA degradation.Molecular and cellular biology · 2004Article
- Identification of a functional core in the RNA component of RNase MRP of budding yeasts.Nucleic acids research · 2004Article
- Genetic changes in the RNA components of RNase MRP and RNase P in Schmid metaphyseal chondrodysplasia.Journal of medical genetics · 2003Article
- Alterations in the intracellular level of a protein subunit of human RNase P affect processing of tRNA precursors.Nucleic acids research · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNase MRP is a ribonucleoprotein (RNP) particle which is involved in the processing of pre-rRNA at site A3 in internal transcribed spacer 1. Although RNase MRP has been analysed functionally, the structure and composition of the particle are not well characterized. A genetic screen for mutants which are synthetically lethal (sl) with a temperature-sensitive (ts) mutation in the RNA component of RNase MRP (rrp2-1) identified an essential gene, POP3, which encodes a basic protein of 22.6 kDa predicted molecular weight. Over-expression of Pop3p fully suppresses the ts growth phenotype of the rrp2-1 allele at 34 degrees C and gives partial suppression at 37 degrees C. Depletion of Pop3p in vivo results in a phenotype characteristic of the loss of RNase MRP activity; A3 cleavage is inhibited, leading to under-accumulation of the short form of the 5.8S rRNA (5.8S(S)) and formation of an aberrant 5.8S rRNA precursor which is 5'-extended to site A2. Pop3p depletion also inhibits pre-tRNA processing; tRNA primary transcripts accumulate, as well as spliced but 5'- and 3'-unprocessed pre-tRNAs. The Pop3p depletion phenotype resembles those previously described for mutations in components of RNase MRP and RNase P (rrp2-1, rpr1-1 and pop1-1). Immunoprecipitation of epitope-tagged Pop3p co-precipitates the RNA components of both RNase MRP and RNase P. Pop3p is, therefore, a common component of both RNPs and is required for their enzymatic functions in vivo. The ubiquitous RNase P RNP, which has a single protein component in Bacteria and Archaea, requires at least two protein subunits for its function in eukaryotic cells.
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