ArticleThe EMBO journal1997
The Xenopus RNA polymerase I transcription factor, UBF, has a role in transcriptional enhancement distinct from that at the promoter.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 28 citations in OpenAlex.
- Nucleolar organizer regions: genomic 'dark matter' requiring illumination.Genes & development · 2016Review
- Construction of synthetic nucleoli and what it tells us about propagation of sub-nuclear domains through cell division.Cell cycle (Georgetown, Tex.) · 2014Article
- Involvement of SIRT7 in resumption of rDNA transcription at the exit from mitosis.Journal of cell science · 2009Article
- RNA-polymerase-I-directed rDNA transcription, life and works.Trends in biochemical sciences · 2005Review
- UBF-binding site arrays form pseudo-NORs and sequester the RNA polymerase I transcription machinery.Genes & development · 2005Article
- UBF binding in vivo is not restricted to regulatory sequences within the vertebrate ribosomal DNA repeat.Molecular and cellular biology · 2002Article
- Transcription and tyranny in the nucleolus: the organization, activation, dominance and repression of ribosomal RNA genes.The arabidopsis book · 2002Article
- Survey and summary: transcription by RNA polymerases I and III.Nucleic acids research · 2000Review
- Dimerization and HMG box domains 1-3 present in Xenopus UBF are sufficient for its role in transcriptional enhancement.Nucleic acids research · 1998Article
- Nucleosome binding by the polymerase I transactivator upstream binding factor displaces linker histone H1.Molecular and cellular biology · 1997Article
- The repeat organizer, a specialized insulator element within the intergenic spacer of the Xenopus rRNA genes.Molecular and cellular biology · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Repeated sequence elements found upstream of the ribosomal gene promoter in Xenopus function as RNA polymerase I-specific transcriptional enhancers. Here we describe an in vitro system in which these enhancers function in many respects as in vivo. The principal requirement for enhancer function in vitro is the presence of a high concentration of upstream binding factor (UBF). This system is utilized to demonstrate that enhancers function by increasing the probability of a stable transcription complex forming on the adjacent promoter. Species differences in UBF are utilized to demonstrate that enhancers do not act by recruiting UBF to the promoter, rather UBF performs its own distinct role at the enhancers. UBF function in enhancement differs from that at the promoter, as it is flexible with respect to both the species of UBF and the enhancer element employed. Additionally, we identify a potential role for the mammalian UBF splice variant, UBF2, in enhancer function. We demonstrate that the TATA box binding protein (TBP)-containing component of Xenopus RNA polymerase I transcription, Rib1, can interact with an enhancer-UBF complex. This suggests a model in which enhancers act by recruiting Rib1 to the promoter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.