ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology1996
Paternally inherited chromosomal structural aberrations detected in mouse first-cleavage zygote metaphases by multicolour fluorescence in situ hybridization painting.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 25 citations in OpenAlex.
- Chromosomal mosaicism in mouse two-cell embryos after paternal exposure to acrylamide.Toxicological sciences : an official journal of the Society of Toxicology · 2009Article
- Fluorescent in situ hybridization for sex chromosome determination before and after fertilization in mice.Theriogenology · 2007Article
- Assessing human germ-cell mutagenesis in the Postgenome Era: a celebration of the legacy of William Lawson (Bill) Russell.Environmental and molecular mutagenesis · 2007Article
- Etoposide induces heritable chromosomal aberrations and aneuploidy during male meiosis in the mouse.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Workshop to identify critical windows of exposure for children's health: reproductive health in children and adolescents work group summary.Environmental health perspectives · 2000Review
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Authors and funding
5 authors at 2 institutions in 1 country.
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Abstract
We describe a fluorescence in situ hybridization (FISH) procedure for assessing zygotic risk of paternal exposure to endogenous or exogenous agents. The procedure employs multicolour FISH with chromosome-specific DNA painting probes plus DAPI staining for detecting both balanced and unbalanced chromosomal aberrations in mouse first-cleavage (1-Cl) zygote metaphases. Four composite probes specific for chromosomes 1, 2, 3 or X, each labelled with biotin, plus a composite probe specific for chromosome Y labelled with digoxigenin, were used. We applied this method to evaluate the effects of paternal exposure to acrylamide, a model germ cell clastogen. First-cleavage zygote metaphases, collected from untreated females mated to males whose sperm or late spermatids were treated with acrylamide, were scored for the induction of structural aberrations using both chromosome painting (PAINT analysis) and DAPI analysis. Structural chromosomal aberrations were observed in the sperm-derived, but not in the egg-derived, pronuclei. While 59.4% of the zygotes had structural aberrations by DAPI analysis, 94.1% of the same zygotes had structural aberrations by PAINT analysis (P < 0.001), illustrating the increased sensitivity for detecting translocations and insertions obtained by adding chromosome painting. These findings show that FISH painting of mouse 1-Cl zygotes when used in conjunction with DAPI analysis is a powerful model for investigating the cytogenetic defects transmitted from father to offspring.
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