ArticleApplied and environmental microbiology1997
Cloning of cellobiose phosphoenolpyruvate-dependent phosphotransferase genes: functional expression in recombinant Escherichia coli and identification of a putative binding region for disaccharides.
Article in Applied and environmental microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 43 citations in OpenAlex.
- An adapted method for Cas9-mediated editing reveals the species-specific role of β-glucoside utilization driving competition betweenJournal of bacteriology · 2024Article
- Identification of Glycoside Transporters From the Human Gut Microbiome.Frontiers in microbiology · 2022Article
- Catabolism of aromatic β-glucosides by bacteria can lead to antibiotics resistance.Archives of microbiology · 2020Article
- Article
- A constitutive expression system for cellulase secretion in Escherichia coli and its use in bioethanol production.PloS one · 2015Article
- Dual substrate specificity of an N-acetylglucosamine phosphotransferase system in Clostridium beijerinckii.Applied and environmental microbiology · 2013Article
- Pectin-rich biomass as feedstock for fuel ethanol production.Applied microbiology and biotechnology · 2012Review
- Addition of genes for cellobiase and pectinolytic activity in Escherichia coli for fuel ethanol production from pectin-rich lignocellulosic biomass.Applied and environmental microbiology · 2011Article
- The PTS transporters of Lactobacillus gasseri ATCC 33323.BMC microbiology · 2010Article
- Homolactic fermentation from glucose and cellobiose using Bacillus subtilis.Microbial cell factories · 2009Article
- How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.Microbiology and molecular biology reviews : MMBR · 2006Review
- Signature-tagged mutagenesis of Klebsiella pneumoniae to identify genes that influence biofilm formation on extracellular matrix material.Infection and immunity · 2006Article
- Mutational analysis of beta-glucoside utilization in Klebsiella aerogenes: evidence for the presence of multiple genetic systems.Journal of genetics · 2004Article
- Cloning, characterization, and functional expression of the Klebsiella oxytoca xylodextrin utilization operon (xynTB) in Escherichia coli.Applied and environmental microbiology · 2003Article
- Gene integration and expression and extracellular secretion of Erwinia chrysanthemi endoglucanase CelY (celY) and CelZ (celZ) in ethanologenic Klebsiella oxytoca P2.Applied and environmental microbiology · 2001Article
- Chromosomal integration of heterologous DNA in Escherichia coli with precise removal of markers and replicons used during construction.Journal of bacteriology · 1999Article
- Enhancement of expression and apparent secretion of Erwinia chrysanthemi endoglucanase (encoded by celZ) in Escherichia coli B.Applied and environmental microbiology · 1999Article
- Isolation and molecular characterization of high-performance cellobiose-fermenting spontaneous mutants of ethanologenic Escherichia coli KO11 containing the Klebsiella oxytoca casAB operon.Applied and environmental microbiology · 1997Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genomic libraries from nine cellobiose-metabolizing bacteria were screened for cellobiose utilization. Positive clones were recovered from six libraries, all of which encode phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) proteins. Clones from Bacillus subtilis, Butyrivibrio fibrisolvens, and Klebsiella oxytoca allowed the growth of recombinant Escherichia coli in cellobiose-M9 minimal medium. The K. oxytoca clone, pLOI1906, exhibited an unusually broad substrate range (cellobiose, arbutin, salicin, and methylumbelliferyl derivatives of glucose, cellobiose, mannose, and xylose) and was sequenced. The insert in this plasmid encoded the carboxy-terminal region of a putative regulatory protein, cellobiose permease (single polypeptide), and phospho-beta-glucosidase, which appear to form an operon (casRAB). Subclones allowed both casA and casB to be expressed independently, as evidenced by in vitro complementation. An analysis of the translated sequences from the EIIC domains of cellobiose, aryl-beta-glucoside, and other disaccharide permeases allowed the identification of a 50-amino-acid conserved region. A disaccharide consensus sequence is proposed for the most conserved segment (13 amino acids), which may represent part of the EIIC active site for binding and phosphorylation.
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