ArticleJournal of bacteriology1997
Identification and characterization of the dTDP-rhamnose biosynthesis and transfer genes of the lipopolysaccharide-related rfb locus in Leptospira interrogans serovar Copenhageni.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 68 citations in OpenAlex.
- Shaping the future ofJournal of medical microbiology · 2025Review
- Salmonella enteritidis acquires phage resistance through a point mutation in rfbD but loses some of its environmental adaptability.Veterinary research · 2024Article
- Genetic structure and diversity of theLife science alliance · 2024Article
- Horizontal transfer of theLife science alliance · 2023Article
- Insight into the Structure, Functions, and Dynamics of theMembranes · 2022Review
- Distinct transcriptional profiles of Leptospira borgpetersenii serovar Hardjo strains JB197 and HB203 cultured at different temperatures.PLoS neglected tropical diseases · 2021Article
- Comparative genomic analysis of Chinese human leptospirosis vaccine strain and circulating isolate.Human vaccines & immunotherapeutics · 2020Article
- Creating a Library of Random Transposon Mutants in Leptospira.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Comparative analysis reveals the Genomic Islands in Pasteurella multocida population genetics: on Symbiosis and adaptability.BMC genomics · 2019Article
- PathogenicOpen veterinary journal · 2018Review
- Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock.BMC veterinary research · 2017Article
- Genomic Analysis of a New Serovar ofFrontiers in microbiology · 2017Article
- Evidence for the horizontal transfer of an unusual capsular polysaccharide biosynthesis locus in marine bacteria.Infection and immunity · 2010Article
- Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen.Nature reviews. Microbiology · 2009Review
- Genetic diversity among major endemic strains of Leptospira interrogans in China.BMC genomics · 2007Article
- Proteins encoded by Sphingomonas elodea ATCC 31461 rmlA and ugpG genes, involved in gellan gum biosynthesis, exhibit both dTDP- and UDP-glucose pyrophosphorylase activities.Applied and environmental microbiology · 2005Article
- Changes in lipopolysaccharide O antigen distinguish acute versus chronic Leptospira interrogans infections.Infection and immunity · 2005Article
- 2F3 monoclonal antibody recognizes the O26 O-antigen moiety of the lipopolysaccharide of enterohemorrhagic Escherichia coli strain 4276.Clinical and diagnostic laboratory immunology · 2004Article
- Genome features of Leptospira interrogans serovar Copenhageni.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2004Article
- Genetic organization of chromosomal S-layer glycan biosynthesis loci of Bacillaceae.Glycoconjugate journal · 2004Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immunity to leptospirosis is principally humorally mediated and involves opsonization of leptospires for phagocytosis by macrophages and neutrophils. The only protective antigen identified to date is the leptospiral lipopolysaccharide (LPS), which biochemically resembles typical gram-negative LPS but has greatly reduced endotoxic activity. Little is known about the structure of leptospiral LPS. A 2.1-kb EcoRI fragment from the chromosome of serovar Copenhageni was cloned in pUC18 in Escherichia coli, after which flanking regions were cloned from a genomic library constructed in bacteriophage lambda GEM12. Sequence analysis identified four open reading frames which showed similarity to the rfbC, rfbD, rfbB, and rfbA genes, transcribed in that order, which encode the four enzymes involved in the biosynthesis of dTDP-rhamnose for the assembly of LPS in Salmonella enterica, E. coli, and Shigella flexneri. An additional open reading frame downstream of the rfbCDBA locus showed similarity with the rhamnosyltransferase genes of Shigella and Yersinia enterocolitica but not Salmonella. Comparison of deduced amino acid sequences showed up to 85% similarity of the leptospiral proteins with those of other gram-negative bacteria. Polyacrylamide gel electrophoresis of recombinant clones identified the putative RfbCDBA proteins, while reverse transcriptase-mediated PCR analysis indicated that the rfbCDBA gene cluster was expressed in Leptospira. Moreover, it could restore normal LPS phenotype to a defined rfbB::Tn5 mutant of S. flexneri which was deficient in all four genes, thereby confirming the functional identification of a part of the leptospiral rfb locus.
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