ArticleJournal of bacteriology1997
The lipoprotein VirB7 interacts with VirB9 in the membranes of Agrobacterium tumefaciens.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
50 citing papers in PubMed, 97 citations in OpenAlex.
- Protein-Protein Interactions: Yeast Two Hybrid.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The VirB System Plays a Crucial Role inInternational journal of molecular sciences · 2021Review
- Architecture of the outer-membrane core complex from a conjugative type IV secretion system.Nature communications · 2021Article
- Two pKM101-encoded proteins, the pilus-tip protein TraC and Pep, assemble on the Escherichia coli cell surface as adhesins required for efficient conjugative DNA transfer.Molecular microbiology · 2019Article
- TraK and TraB are conserved outer membrane proteins of the Neisseria gonorrhoeae Type IV secretion system and are expressed at low levels in wild-type cells.Journal of bacteriology · 2014Article
- Surface organelles assembled by secretion systems of Gram-negative bacteria: diversity in structure and function.FEMS microbiology reviews · 2012Review
- The Gonococcal Genetic Island and Type IV Secretion in the Pathogenic Neisseria.Frontiers in microbiology · 2011Article
- Interaction of variable bacterial outer membrane lipoproteins with brain endothelium.PloS one · 2010Article
- Phylogenomics reveals a diverse Rickettsiales type IV secretion system.Infection and immunity · 2010Review
- The structural biology of type IV secretion systems.Nature reviews. Microbiology · 2009Review
- Agrobacterium VirB10 domain requirements for type IV secretion and T pilus biogenesis.Molecular microbiology · 2009Article
- Structure of a type IV secretion system core complex.Science (New York, N.Y.) · 2009Article
- Article
- SciN is an outer membrane lipoprotein required for type VI secretion in enteroaggregative Escherichia coli.Journal of bacteriology · 2008Article
- The type IV secretion system component VirB5 binds to the trans-zeatin biosynthetic enzyme Tzs and enables its translocation to the cell surface of Agrobacterium tumefaciens.Journal of bacteriology · 2008Article
- VirB1* promotes T-pilus formation in the vir-Type IV secretion system of Agrobacterium tumefaciens.Journal of bacteriology · 2007Article
- NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Detection of conjugation related type four secretion machinery in Aeromonas culicicola.PloS one · 2006Article
- Protein-protein interactions among Helicobacter pylori cag proteins.Journal of bacteriology · 2006Article
- The Brucella suis type IV secretion system assembles in the cell envelope of the heterologous host Agrobacterium tumefaciens and increases IncQ plasmid pLS1 recipient competence.Infection and immunity · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
VirB9 and VirB7 are essential components of the putative VirB membrane channel required for transfer of the T-complex from Agrobacterium tumefaciens into plants. In this report, we present a biochemical analysis of their interaction and cellular localization. A comparison of relative electrophoretic mobilities under nonreducing and reducing conditions suggested that they form thiol-sensitive complexes with other proteins. Two-dimensional gel electrophoresis identified one complex as a heterodimer of VirB9 and VirB7 covalently linked by a disulfide bond, as well as VirB7 homodimers and monomers. Immunoprecipitation with VirB9-specific antiserum isolated the heterodimeric VirB9-VirB7 complex. Incubation with reducing agent split the complex into its constituent VirB9 and VirB7, which further confirmed linkage via cysteine residues. The interaction between VirB9 and VirB7 also was observed in the yeast two-hybrid system. Membrane attachment of VirB9-VirB7 may be conferred by lipoprotein modification, since labeling with [3H]palmitic acid in A. tumefaciens verified that VirB7 is a lipoprotein associated with VirB9. VirB9 and VirB7 showed equal distribution between inner and outer membranes, in accord with their proposed association with the transmembrane VirB complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.