ArticleNucleic acids research1997
Intronic and exonic sequences modulate 5' splice site selection in plant nuclei.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Rock, scissors, paper: How RNA structure informs function.The Plant cell · 2023Article
- Comparative studies of Toxoplasma gondii transcriptomes: insights into stage conversion based on gene expression profiling and alternative splicing.Parasites & vectors · 2018Article
- Exploring the relationship between intron retention and chromatin accessibility in plants.BMC genomics · 2018Article
- Development of an in vitro pre-mRNA splicing assay using plant nuclear extract.Plant methods · 2018Article
- Germination Potential of Dormant and Nondormant Arabidopsis Seeds Is Driven by Distinct Recruitment of Messenger RNAs to Polysomes.Plant physiology · 2015Article
- Genome-Wide Analysis of Heat-Sensitive Alternative Splicing in Physcomitrella patens.Plant physiology · 2014Article
- Genome-wide analysis of light-regulated alternative splicing mediated by photoreceptors in Physcomitrella patens.Genome biology · 2014Article
- Deciphering the plant splicing code: experimental and computational approaches for predicting alternative splicing and splicing regulatory elements.Frontiers in plant science · 2012Article
- Transcriptional and post-transcriptional enhancement of gene expression by the 5' UTR intron of rice rubi3 gene in transgenic rice cells.Molecular genetics and genomics : MGG · 2008Article
- Expression enhancement of a rice polyubiquitin gene promoter.Plant molecular biology · 2006Article
- Evaluation of Glycine max mRNA clusters.BMC bioinformatics · 2005Article
- Surrogate splicing for functional analysis of sesquiterpene synthase genes.Plant physiology · 2005Article
- Depletion of TDP 43 overrides the need for exonic and intronic splicing enhancers in the human apoA-II gene.Nucleic acids research · 2005Article
- Determinants of plant U12-dependent intron splicing efficiency.The Plant cell · 2004Article
- Transcription-coupled and splicing-coupled strand asymmetries in eukaryotic genomes.Nucleic acids research · 2004Article
- Nucleotide frequency variation across human genes.Genome research · 2003Article
- Article
- Distribution and characterization of regulatory elements in the human genome.Genome research · 2002Article
- Splicing of the maize Sh1 first intron is essential for enhancement of gene expression, and a T-rich motif increases expression without affecting splicing.Plant physiology · 2002Article
- AT-AC pre-mRNA splicing mechanisms and conservation of minor introns in voltage-gated ion channel genes.Molecular and cellular biology · 1999Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Pre-mRNA transcripts in a variety of organisms, including plants, Drosophila and Caenorhabditis elegans, contain introns which are significantly richer in adenosine and uridine residues than their flanking exons. Previous analyses using exonic and intronic replacements between two nonequivalent 5'splice sites in the 469 nt long rbcS3A intron 1 provided the first evidence indicating that, in both tobacco and Drosophila nuclei, 5'splice site selection is strongly influenced by the position of that site relative to the AU transition point between exon and intron. To differentiate between two potential models for 5'splice site recognition, we have expressed a completely different set of intronic and exonic replacement constructs containing identical 5'splice sites upstream of beta-conglycinin intron 4 (115 nt). Mutagenesis and deletion of the upstream 5'splice site demonstrate that intronic AU-rich sequences function by promoting recognition of the most upstream 5'splice site rather than by masking the downstream 5'splice site. Sequence insertions define a role for AG-rich exonic sequences in plant pre-mRNA splicing by demonstrating that an AG-rich element is capable of promoting downstream 5'splice site recognition. We conclude that AU-rich intronic sequences, AG-rich exonic sequences and the 5'splice site itself collectively define 5'intron boundaries in dicot nuclei.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.