ArticleNucleic acids research1997
Characterization of multigene families in the micronuclear genome of Paramecium tetraurelia reveals a germline specific sequence in an intron of a centrin gene.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed, 17 citations in OpenAlex.
- Developmentally regulated excision of a 28-base-pair sequence from the Paramecium genome requires flanking DNA.Molecular and cellular biology · 2000Article
- Timing of developmentally programmed excision and circularization of Paramecium internal eliminated sequences.Molecular and cellular biology · 2000Article
- Article
- Homology-dependent maternal inhibition of developmental excision of internal eliminated sequences in Paramecium tetraurelia.Molecular and cellular biology · 1998Article
- Homology-dependent gene silencing in Paramecium.Molecular biology of the cell · 1998Article
- A mutation in Paramecium tetraurelia reveals functional and structural features of developmentally excised DNA elements.Genetics · 1998Article
- Deletion endpoint allele-specificity in the developmentally regulated elimination of an internal sequence (IES) in Paramecium.Nucleic acids research · 1997Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Paramecium, as in other ciliates, the transcriptionally active macronucleus is derived from the germline micronucleus by programmed DNA rearrangements, which include the precise excision of thousands of germline-specific sequences (internal eliminated sequences, IESs). We report the characterization of micronuclear versions of genes encoding Paramecium secretory granule proteins (trichocyst matrix proteins, TMPs) and Paramecium centrins. TMP and centrin multigene families, previously studied in the macronuclear genome, consist of genes that are co-expressed to provide mixtures of related polypeptides that co-assemble to form respectively the crystalline trichocyst matrix and the infraciliary lattice, a contractile cytoskeletal network. We present evidence that TMP and centrin genes identified in the macronucleus are also present in the micronucleus, ruling out the possibility that these novel multigene families are generated by somatic rearrangements during macronuclear development. No IESs were found in TMP genes, however, four IESs in or near germline centrin genes were characterized. The only intragenic IES is 75 bp in size, interrupts a 29 bp intron and is absent from at least one other closely related centrin gene. This is the first report of an IES in an intron in Paramecium.
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