ReviewJournal of clinical laboratory analysis1997
In situ hybridization: methods and applications.
Review in Journal of clinical laboratory analysis, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed.
- Optimizing the hybridization chain reaction-fluorescence in situ hybridization (HCR-FISH) protocol forbioRxiv : the preprint server for biology · 2026Article
- HyperSTAR: Unveiling Tissue Structure and Tumor Microenvironment from Spatial Omics by Hypergraph Learning.Genomics, proteomics & bioinformatics · 2026Article
- Streamlined Full-Length Total RNA Sequencing of Paraformaldehyde-Fixed Brain Tissues.International journal of molecular sciences · 2024Article
- Sopa: a technology-invariant pipeline for analyses of image-based spatial omics.Nature communications · 2024Article
- Role ofInternational journal of molecular sciences · 2024Article
- Review
- Insights into brain tumor diagnosis: exploringFrontiers in neurology · 2024Review
- A Novel Method for In Situ RT-PCR Based on Capsules from Centrifuge Tubes, Ideal for Transcripts Detection in Plant Tissues.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Recent Advances in High-sensitivityActa histochemica et cytochemica · 2023Review
- Isthmin-1 (Ism1) modulates renal branching morphogenesis and mesenchyme condensation during early kidney development.Nature communications · 2023Article
- Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The Protocol of Circulating Tumor Cell Detection.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Colorimetric Whole-Mount In Situ Hybridization in Planarians.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Analysis of Spatial Gene Expression at the Cellular Level in Stony Corals.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Review
- MicroRNAs Regulating Autophagy in Neurodegeneration.Advances in experimental medicine and biology · 2021Article
- Detection of SARS-CoV-2 by RNAscopeArchives of virology · 2020Article
- Integration of molecular tools in microbial phosphate solubilization research in agriculture perspective.World journal of microbiology & biotechnology · 2020Review
- Recent Advances in Molecular Diagnostics of Fungal Plant Pathogens: A Mini Review.Frontiers in cellular and infection microbiology · 2020Review
- All-age whole mount in situ hybridization to reveal larval and juvenile expression patterns in zebrafish.PloS one · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In situ hybridization (ISH) combines molecular biological techniques with histological and cytological analysis of gene expression. RNA and DNA can be readily localized in specific cells with this method. ISH has been useful as a research tool, and recent studies have used this technique in the diagnostic pathology laboratory and in microbiology for the tissue localization in infectious agents. Other recent developments in the applications of ISH involve in situ polymerase chain reaction (PCR) and in situ reverse transcription (RT)-PCR, which can be used to detect very low levels of nucleic acids in tissues by taking advantage of the powerful amplification capacity of PCR. In situ PCR will contribute significantly to progress in this field because of the marked increase in the sensitivity of this method.
Indexed as
Identifiers
9021518PMC6760707What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.