ArticleThe Biochemical journal1997
Ras activation in platelets after stimulation of the thrombin receptor, thromboxane A2 receptor or protein kinase C.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 42 citations in OpenAlex.
- A chemigenetic endogenous Ras activity biosensor for live-cell pharmacology.Nature chemical biology · 2026Article
- Characterisation of the Ral GTPase inhibitor RBC8 in human and mouse platelets.Cellular signalling · 2019Article
- Small GTPases in platelet membrane trafficking.Platelets · 2019Review
- RAP GTPases and platelet integrin signaling.Platelets · 2019Review
- TC21/RRas2 regulates glycoprotein VI-FcRγ-mediated platelet activation and thrombus stability.Journal of thrombosis and haemostasis : JTH · 2018Article
- The Phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P3) Binder Rasa3 Regulates Phosphoinositide 3-kinase (PI3K)-dependent Integrin αIIbβ3 Outside-in Signaling.The Journal of biological chemistry · 2017Article
- RASA3 is a critical inhibitor of RAP1-dependent platelet activation.The Journal of clinical investigation · 2015Article
- Platelet function and Isoprostane biology. Should isoprostanes be the newest member of the orphan-ligand family?Journal of biomedical science · 2010Review
- Article
- Thromboxane A2 is a key regulator of pathogenesis during Trypanosoma cruzi infection.The Journal of experimental medicine · 2007Article
- Low-density lipoprotein activates the small GTPases Rap1 and Ral in human platelets.The Biochemical journal · 2000Article
- Farnesylcysteine analogues inhibit store-regulated Ca2+ entry in human platelets: evidence for involvement of small GTP-binding proteins and actin cytoskeleton.The Biochemical journal · 2000Article
- R-Ras signals through specific integrin alpha cytoplasmic domains to promote migration and invasion of breast epithelial cells.The Journal of cell biology · 1999Article
- Activation of the small GTPase Ral in platelets.Molecular and cellular biology · 1998Article
- Affinity modulation of platelet integrin alphaIIbbeta3 by beta3-endonexin, a selective binding partner of the beta3 integrin cytoplasmic tail.The Journal of cell biology · 1997Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Several reports have indicated that the small G-protein Ras is not present immunologically in platelets. However, here we report the identification of Ras in platelets by immunoprecipitation with the Ras-specific monoclonal antibodies Y13-259 or Y13-238, followed by Western blotting. The presence of Ras was not due to contamination of samples with erythrocytes or leucocytes. Immunofluorescence studies indicated that Ras was present in a peripheral rim pattern in fixed, permeabilized platelets, suggesting an intracellular, plasma membrane location. Activation of platelets with the thrombin receptor peptide42-50, the prostaglandin H2/thromboxane A2 mimetic U46619 or phorbol 12-myristate 13-acetate induced a rapid increase in GTP-bound, activated Ras. In each case, this increase was inhibited by the protein kinase C (PKC) inhibitor bisindolylmaleimide GF 109203X, suggesting that Ras is activated downstream of PKC in platelets. Thus the activation of Ras in platelets by agonists will now allow consideration of multiple potential Ras-dependent signal transduction pathways in platelet activation processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.