ArticleThe Biochemical journal1997
The beta-D-xylosidase of Trichoderma reesei is a multifunctional beta-D-xylan xylohydrolase.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 103 citations in OpenAlex.
- Cellulose Degradation Enzymes in Filamentous Fungi, A Bioprocessing Approach Towards Biorefinery.Molecular biotechnology · 2025Review
- Use of xylosidase 3C from Segatella baroniae to discriminate xylan non-reducing terminus substitution characteristics.BMC research notes · 2024Article
- Differential Carbon Catabolite Repression and Hemicellulolytic Ability among Pathotypes ofJournal of fungi (Basel, Switzerland) · 2024Article
- Elimination of the Sugar Transporter GAT1 Increased Xylanase I Production inFrontiers in microbiology · 2022Article
- Non-Specific GH30_7 Endo-β-1,4-xylanase fromMolecules (Basel, Switzerland) · 2021Article
- A novel bacterial GH30 xylobiohydrolase from Hungateiclostridium clariflavum.Applied microbiology and biotechnology · 2021Article
- β-Xylosidases: Structural Diversity, Catalytic Mechanism, and Inhibition by Monosaccharides.International journal of molecular sciences · 2019Review
- The Change in the Composition ofMicrobiology insights · 2019Article
- Ruminal metagenomic libraries as a source of relevant hemicellulolytic enzymes for biofuel production.Microbial biotechnology · 2018Article
- Article
- Article
- Purification and characterization of an extracellular β-xylosidase from Pseudozyma hubeiensis NCIM 3574 (PhXyl), an unexplored yeast.AMB Express · 2016Article
- Production of β-xylosidase from Trichoderma asperellum KIF125 and its application in efficient hydrolysis of pretreated rice straw with fungal cellulase.World journal of microbiology & biotechnology · 2016Article
- Novel pH-Stable Glycoside Hydrolase Family 3 β-Xylosidase from Talaromyces amestolkiae: an Enzyme Displaying Regioselective Transxylosylation.Applied and environmental microbiology · 2015Article
- Highly thermostable GH39 β-xylosidase from a Geobacillus sp. strain WSUCF1.BMC biotechnology · 2014Article
- Construction of a promoter collection for genes co-expression in filamentous fungus Trichoderma reesei.Journal of industrial microbiology & biotechnology · 2014Article
- Production of intracellular β-xylosidase from the submerged fermentation of citrus wastes by Penicillium janthinellum MTCC 10889.3 Biotech · 2013Article
- Regulation of cellulase and hemicellulase gene expression in fungi.Current genomics · 2013Article
- Purification and characterization of a recombinant β-D-xylosidase from Thermobifida fusca TM51.The protein journal · 2012Article
- A novel xylan degrading β-D-xylosidase: purification and biochemical characterization.World journal of microbiology & biotechnology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An extracellular multifunctional beta-D-xylan xylohydrolase, previously described as beta-xylosidase, was purified from Trichoderma reesei RUT C-30 to physical homogeneity. The active enzyme was a 100 (+/-5) kDa glycosylated monomer that exhibited a pl of 4.7. Its activity was optimal at pH 4 and it was stable between pH 3 and 6. Its temperature-stability was moderate (70 degrees zero of activity remaining after 60 min at 50 degrees C) and optimal activity was observed at 60 degrees C. It is capable of hydrolysing beta-1.4-xylo-oligosaccharides [degree of polymerization (DP) 2-7], the apparent Vmax increasing with increasing chain length. The enzyme also attacked debranched beech-wood (Lenzing) xylan and 4-O-methylglucuronoxylan, forming xylose as the only end product. The K(m) for xylan was 0.7 g/l. For this reason we consider the enzyme to be a beta-D-xylan xylohydrolase. The enzyme also exhibits alpha-L-arabinofuranosidase activity on 4-nitrophenyl alpha-L-arabinofuranoside, and evidence is presented that this is not caused by an impurity in the enzyme preparation. The beta-D-xylan xylohydrolase exhibits glycosyltransferase activity with xylo-oligosaccharides and at high concentrations of 4-nitrophenyl beta-D-xylopyranoside (4-Nph-beta-Xyl). The enzyme hydrolyses beta-1, 4-linkages preferentially to beta-1,3-linkages, and beta-1,2-linked xylo-oligosaccharides are not hydrolysed at all. The enzyme liberates terminal beta-1,4-xylopyranose residues linked to a 2-O-substituted xylopyranose residue, but not that linked to a 3-O-substituted xylopyranose residue. The enzyme does not attack methyl, methyl 1-thio-benzyl or butyl l-thio-beta-D-xylopyranosides and 4-naphthyl, 2-naphthyl and phenyl beta-D-xylopyranosides.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.